| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,808,040 | A→G | 15.9% | intergenic (+119/+36) | mrcA → / ← maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,808,040 | 0 | A | G | 15.9% | 55.8 / 2.7 | 25 | intergenic (+119/+36) | mrcA/maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Reads supporting (aligned to +/- strand): ref base A (12/9); new base G (2/2); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GATGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGA > NC_002947/5807892‑5808182 | gATGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCGAAGCCACATAACACAGCCCCGGCTCAt < 2:19004/150‑1 (MQ=255) ttCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGGTTTTTTATgggg > 1:226851/1‑147 (MQ=38) aGGCTGTGCGCCTGACTCAGGTTCTGCGTGGGCGCTCGTTCTGATAGGCGCCACAAGCGCCCTACCCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTtgg < 2:226851/150‑2 (MQ=25) gCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtgctg > 2:291874/1‑150 (MQ=255) aGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCa > 2:10448/1‑150 (MQ=255) tCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGc < 1:253139/150‑1 (MQ=255) cacaAGTGCCCTGACCACCACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACCAGGCGGCCACGCTGGTCAGCGGGTAATGCTTc < 1:12717/150‑1 (MQ=255) cTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCa > 2:457663/1‑150 (MQ=255) cTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACCCTGGTCAGCGGGTAATGATTCGGATAAGGCa > 1:464145/1‑150 (MQ=255) aaCACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAg > 2:480247/1‑150 (MQ=255) cTTGCAGCCCTGACACATTCCCAAGCCCAATAAACAAGCACAGGAACACCAGAGCCGGGGCTTTTTTATGGCTCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCAGGTAATGCTTCGGATAAGGCAGGGTAGCCACg > 1:331426/1‑150 (MQ=255) cccTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGAtt < 1:10448/150‑1 (MQ=255) ccggACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTc < 2:52254/147‑1 (MQ=255) aTTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAg > 2:200824/1‑150 (MQ=255) tCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCCGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGCTGGCAGcc < 1:480247/150‑1 (MQ=255) cATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg < 1:363721/83‑1 (MQ=255) cATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg > 2:363721/1‑83 (MQ=255) aTAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTaa < 1:364278/94‑1 (MQ=25) aTAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTaa > 2:364278/1‑94 (MQ=25) aaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGc > 1:201591/1‑112 (MQ=255) aaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGc < 2:201591/112‑1 (MQ=255) ccccGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAac > 2:479202/1‑150 (MQ=255) cccGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc < 1:79535/90‑1 (MQ=255) cccGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc > 2:79535/1‑90 (MQ=255) ccGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacga > 1:337314/1‑150 (MQ=255) | GATGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGA > NC_002947/5807892‑5808182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |