Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,815,592 G→T 10.0% intergenic (+20/+71) PP_5090 → / ← PP_5091 cell division protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,815,5920GT10.0% 112.4 / 3.1 40intergenic (+20/+71)PP_5090/PP_5091cell division protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base T (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

AACCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCCCACCAGGAA  >  NC_002947/5815449‑5815737
                                                                                                                                               |                                                                                                                                                 
aaCCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGc                                                                                                                                             <  1:413076/150‑1 (MQ=255)
  ccTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGa                                                                                                                                           >  2:191429/1‑150 (MQ=255)
     ggTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTCACAGCGGCACACAAGCCGCTGGCTGGGGCCGGGCTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACaa                                                                                                                                        <  1:478794/150‑1 (MQ=255)
      gcaacgcgTGCTGGTTGGGCCGTCCAGCAACCGCGAGCAGTTGACAGGGGAACAGAAGCAGGTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCAGCGCTGCGGCAAg                                                                                                                                       <  2:222196/146‑1 (MQ=255)
               gCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCAAGGCcttcttc                                                                                                                              <  1:327858/150‑2 (MQ=39)
                 tGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTGAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTtat                                                                                                                            >  2:329840/1‑150 (MQ=255)
                      gggCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCaa                                                                                                                                        >  1:464246/1‑133 (MQ=255)
                      gggCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGTCGCATCGCTGCGGCaa                                                                                                                                        <  2:464246/133‑1 (MQ=255)
                                   aCCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGt                                                                                                          <  1:290224/150‑1 (MQ=255)
                                   aCCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTAAACAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGt                                                                                                          >  1:334954/1‑150 (MQ=255)
                                    ccGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTg                                                                                                         >  2:230732/1‑150 (MQ=255)
                                     cgcgAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAg                                                                                                                                       <  2:223024/119‑1 (MQ=255)
                                     cgcgAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAg                                                                                                                                       >  1:223024/1‑119 (MQ=255)
                                     cgcgAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGa                                                                                                        >  1:472255/1‑150 (MQ=255)
                                                  cAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGAAAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAag                                                                                           <  1:191429/150‑1 (MQ=255)
                                                   aGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagc                                                                                          >  1:210256/1‑150 (MQ=255)
                                                     gggCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagcag                                                                                        <  2:82556/150‑1 (MQ=255)
                                                                    tggctggGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAg                                                                         <  2:142749/150‑1 (MQ=255)
                                                                                ggTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCa                                                             <  2:472255/150‑1 (MQ=255)
                                                                                 gTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCAt                                                            >  2:347658/1‑150 (MQ=255)
                                                                                     aGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTatat                                                                                                                          >  2:261174/1‑84 (MQ=255)
                                                                                     aGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTatat                                                                                                                          <  1:261174/84‑1 (MQ=255)
                                                                                      gCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGccc                                                                                                            <  1:171850/97‑1 (MQ=255)
                                                                                      gCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGccc                                                                                                            >  2:171850/1‑97 (MQ=255)
                                                                                          ccTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCGCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCgg                                                   >  2:223345/1‑150 (MQ=255)
                                                                                          ccTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCgg                                                   >  1:361805/1‑150 (MQ=255)
                                                                                                  tgcAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAgg                                                                                                     >  1:399986/1‑92 (MQ=255)
                                                                                                  tgcAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAgg                                                                                                     <  2:399986/92‑1 (MQ=255)
                                                                                                        aGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCCACCCCCACGAAGCGAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTc                                     >  1:233427/1‑150 (MQ=255)
                                                                                                           ggCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGACGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGtt                                      <  1:438215/146‑1 (MQ=255)
                                                                                                           ggCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGACGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGtt                                      >  2:438215/1‑146 (MQ=255)
                                                                                                                     tCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagca                                                                                         >  2:312367/1‑85 (MQ=255)
                                                                                                                     tCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAagca                                                                                         <  1:312367/85‑1 (MQ=255)
                                                                                                                         tAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCa                             >  2:348973/1‑141 (MQ=255)
                                                                                                                         tAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCa                             <  1:348973/141‑1 (MQ=255)
                                                                                                                                     ccTTGTCGCATCGCTGCGGCAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGTATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCccac        >  1:303658/1‑150 (MQ=255)
                                                                                                                                      cTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCttt                                                      >  1:216375/1‑103 (MQ=255)
                                                                                                                                      cTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCttt                                                      <  2:216375/103‑1 (MQ=255)
                                                                                                                                       ttGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCccacca      >  2:272616/1‑150 (MQ=255)
                                                                                                                                           cGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCCCACCAGGaa  <  2:334954/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AACCTGGTACCGCGTGCTGGTTGGGCCGTTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAGCATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCCCACCAGGAA  >  NC_002947/5815449‑5815737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑