| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,897,462 | T→C | 24.5% | intergenic (‑18/+12) | sbp‑II ← / ← PP_5172 | sulfate ABC transporter substrate‑binding protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,897,462 | 0 | T | C | 24.5% | 20.8 / 2.7 | 16 | intergenic (‑18/+12) | sbp‑II/PP_5172 | sulfate ABC transporter substrate‑binding protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (2/2); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTCGGGTCATAGGACACGTTGAGCAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCTGTACGACCTGAGCGTTGTGCACGGTGATCTCCACCGCACCGAAACGCAGGTCGCGCAGGGCGCTCTGGA > NC_002947/5897318‑5897598 | gggtcgggtcATAGGACACGTTGAGCAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGAGGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTgg > 1:356087/1‑150 (MQ=255) aTAGGACACGTTGAGCAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTTGCCAGGGCTGCGAGCGAATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAg > 1:112893/1‑150 (MQ=255) aGGACACGTTGAGCAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTGGACAGGGCGGAGAGCGAATAATGGAGGATGGAGATGTTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCAGATCacgc > 2:215852/1‑147 (MQ=255) cAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGCTGTGGCGCCGATCAGCCGGGCTTGTTGCCg > 1:242377/1‑150 (MQ=255) ggCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTtc < 1:31519/150‑1 (MQ=255) gCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTtct > 2:355617/1‑150 (MQ=255) aCCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGCTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTg < 2:242377/150‑1 (MQ=255) ggCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTtct > 1:172934/1‑142 (MQ=255) ggCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTtct < 2:172934/142‑1 (MQ=255) aaCAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATc < 1:280496/150‑1 (MQ=255) aCAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCt < 2:112893/150‑1 (MQ=255) aGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGCTGTGGCGCCGATCAGCCg > 1:370612/1‑89 (MQ=255) aGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGCTGTGGCGCCGATCAGCCg < 2:370612/89‑1 (MQ=255) gcgcATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCTGTACGACCTGAGCGTtg < 1:452217/145‑1 (MQ=255) gcgcATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCTGTACGACCTGAGCGTtg > 2:452217/1‑145 (MQ=255) cTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCTGTACGACCTGAGCGTTGTGCACGGTGATCTCCACCGCACCGAAACGCAGGTCGCGCAGGGCGCTCTGGa < 2:356087/150‑1 (MQ=255) | GGGTCGGGTCATAGGACACGTTGAGCAGTTCGTAGTCCTTGGCGATGGCGGAACCGGCAAAAACAGCACTGGCCAGGGCGGCGAGCGCATAACGGCGGATGGACATGGTGAAGCTCCCGATTGGCATTTTTCTAGTTTTGGATGTTGTGGCGCCGATCAGCCGGGCTTGTTGCCGGGCTGTTGCAGGCGGAACTTCTCCTTGCGCTCGATCTGTACGACCTGAGCGTTGTGCACGGTGATCTCCACCGCACCGAAACGCAGGTCGCGCAGGGCGCTCTGGA > NC_002947/5897318‑5897598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |