| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,572 | G→A | 27.3% | K1026K (AAG→AAA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,572 | 0 | G | A | 27.3% | 45.1 / 19.2 | 33 | K1026K (AAG→AAA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (12/12); new base A (5/4); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
CCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCG > NC_002947/197430‑197717 | ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAg > 2:340996/1‑149 (MQ=39)ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAg < 1:340996/149‑1 (MQ=39) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGc > 2:51716/1‑150 (MQ=38) gACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCTTGGGCAATCTTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAATCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCGGCGTCGa > 1:88701/1‑150 (MQ=11) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGAttt > 1:418499/1‑150 (MQ=39) cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCCGACGGCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTc < 2:463099/150‑1 (MQ=255) gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGAcccc < 1:477300/150‑1 (MQ=38) gTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGaa < 1:351638/150‑1 (MQ=39) cggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTAcaacaa < 2:476454/150‑1 (MQ=39) cgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACgg > 2:62754/1‑150 (MQ=38) tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCa < 2:286858/150‑1 (MQ=18) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCAt < 1:51716/150‑1 (MQ=17) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTT‑‑‑GCCGTAACAGTCGTGCACTTCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCAt > 2:142114/1‑150 (MQ=11) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCg > 1:128307/1‑150 (MQ=38) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCg > 1:423440/1‑150 (MQ=17) gAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCga < 1:62754/150‑1 (MQ=39) cAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGc > 2:459625/1‑150 (MQ=39) aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg < 2:373933/150‑1 (MQ=11) aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCg < 1:416698/150‑1 (MQ=17) gACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCgg < 2:418499/150‑1 (MQ=39) gaCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGCAGCCGGCG‑AAAC‑‑GCAGGTCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACACCGGTTCGACTGTCAGCGTAACAATCGAGAACGCCACAGGCGGTATTTTCGa < 2:399172/149‑1 (MQ=11) ccTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc > 2:282579/1‑150 (MQ=12) cTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAcc > 1:48356/1‑150 (MQ=12) tGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc > 1:405224/1‑148 (MQ=32) tGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc < 2:405224/148‑1 (MQ=32) gCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 2:265389/130‑1 (MQ=255) gCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa > 1:265389/1‑130 (MQ=255) gTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAg < 2:120249/103‑1 (MQ=33) gTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAg > 1:120249/1‑103 (MQ=33) aaGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGa > 2:440031/1‑150 (MQ=38) gCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAc < 2:266883/126‑1 (MQ=255) gCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAc > 1:266883/1‑126 (MQ=255) ggCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACC‑TCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCATCg > 1:112218/1‑150 (MQ=11) | CCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCG > NC_002947/197430‑197717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |