Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 72,026 G→A 8.2% R275C (CGT→TGT)  glyQ ← glycine‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294772,0260GA 8.2% 138.1 / 2.9 49R275C (CGT→TGT) glyQglycine‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGTTGGCTTCG  >  NC_002947/71883‑72159
                                                                                                                                               |                                                                                                                                     
ccAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGCGGGCCGCGCGTGCACGGGCTTGCAGATAGCTTTGCGCAAAATCCCGGTCCAGGGTACGTACACGCAGGAt                                                                                                                                 >  1:134044/1‑150 (MQ=255)
ccAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGAt                                                                                                                                 <  2:419596/150‑1 (MQ=255)
ccAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGAt                                                                                                                                 >  2:279392/1‑150 (MQ=255)
   ggAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTa                                                                                                                              <  1:309993/150‑1 (MQ=255)
     aaaTCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGATAAGTCTAGGCGGGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAAc                                                                                                                            >  1:428737/1‑150 (MQ=255)
      aaTCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGGTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACACAGGATGTAACg                                                                                                                           >  1:235699/1‑150 (MQ=255)
        tCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCAGGGCCAGGGTACGTACACGCAGGATGTAACGCt                                                                                                                         >  1:222649/1‑150 (MQ=255)
         cTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTg                                                                                                                        >  1:401119/1‑150 (MQ=255)
          ttGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTgg                                                                                                                       >  1:344847/1‑150 (MQ=255)
          ttGAGCACCAATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTgg                                                                                                                       >  2:30648/1‑150 (MQ=255)
                         gCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCgag                                                                                                        <  1:112155/150‑1 (MQ=255)
                          cAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCgaga                                                                                                       >  2:201128/1‑150 (MQ=255)
                             ccTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATgg                                                                                                    <  1:187237/150‑1 (MQ=255)
                              cTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGgc                                                                                                   >  1:132590/1‑150 (MQ=255)
                                   gCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCgcggc                                                                                              <  1:279392/150‑1 (MQ=255)
                                         ccAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGt                                                                                        >  2:419050/1‑150 (MQ=255)
                                          cAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTc                                                                                       >  2:153204/1‑150 (MQ=255)
                                            acacTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCca                                                                                     >  2:338579/1‑150 (MQ=255)
                                              acTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGt                                                                                                               >  1:103019/1‑122 (MQ=255)
                                              acTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGt                                                                                                               <  2:103019/122‑1 (MQ=255)
                                               cTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGACACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCcagca                                                                                  <  2:222649/150‑1 (MQ=255)
                                                         cAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGt                                                                        <  1:419050/150‑1 (MQ=255)
                                                                      gCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTc                                                           <  2:192222/150‑1 (MQ=255)
                                                                       ccATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCa                                                          <  2:134044/150‑1 (MQ=255)
                                                                        ttgcGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCgcggc                                                                                              <  1:25687/110‑1 (MQ=255)
                                                                        cATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCgcggc                                                                                              >  2:25687/1‑113 (MQ=255)
                                                                        cATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAg                                                         <  2:344847/150‑1 (MQ=255)
                                                                         aTCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGt                                                                        <  2:320600/134‑1 (MQ=255)
                                                                         aTCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGt                                                                        >  1:320600/1‑134 (MQ=255)
                                                                          tCGGGAAGCCCAGGCTTTCGCGGGCGTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACACAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGa                                                       <  2:235699/150‑1 (MQ=255)
                                                                                  cccAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCa                                               >  2:23858/1‑150 (MQ=255)
                                                                                  cccAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACACAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCa                                               >  1:115201/1‑150 (MQ=255)
                                                                                    cAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATa                                             >  1:190425/1‑150 (MQ=255)
                                                                                    cAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACACAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATa                                             <  2:115201/150‑1 (MQ=255)
                                                                                     aGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAg                                            <  1:153204/150‑1 (MQ=255)
                                                                                                   ttGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTcggcagcggcag                              >  1:33773/1‑150 (MQ=255)
                                                                                                   ttGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTcggcagcggcag                              >  2:491903/1‑150 (MQ=255)
                                                                                                    tGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCggcagcggcagc                             <  2:132590/150‑1 (MQ=255)
                                                                                                         aTAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAg                        >  2:352660/1‑150 (MQ=255)
                                                                                                                   gcCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGaa                                                                           <  1:366191/89‑1 (MQ=255)
                                                                                                                   gcCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGc                       <  2:141915/141‑1 (MQ=255)
                                                                                                                   gcCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGc                       >  1:141915/1‑141 (MQ=255)
                                                                                                                   gcCACGTCCCGGGCAAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGaa                                                                           >  2:366191/1‑89 (MQ=255)
                                                                                                                    ccACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCATGc             <  1:491903/150‑1 (MQ=255)
                                                                                                                     cACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCg            <  1:23858/150‑1 (MQ=255)
                                                                                                                        gTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGtt         <  1:338579/150‑1 (MQ=255)
                                                                                                                             gggCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGTTGGCtt    <  2:33773/150‑1 (MQ=255)
                                                                                                                              ggCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGTTGGCTTc   <  1:201128/150‑1 (MQ=255)
                                                                                                                               gCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGACGTATAGGGTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGTTGGCTTCg  <  1:30648/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                     
CCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTTCAGGGGTGGCCATCGGGAAGCCCAGGCGTGCGCGGGCTTGCAGATAGCTTTGCGCCACGTCCCGGGCCAGGGTACGTACACGCAGGATGTAACGCTGGCGCTCGGTTACCGAGATGGCGCGGCGGGCGTCCAGCAGGTTGAAGGTATGCGATGCCTTCAGGACCATTTCATAGGTCGGCAGCGGCAGCTCCAGCTTGATCAGGCGGTTGGCTTCG  >  NC_002947/71883‑72159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑