| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 76,882 | G→C | 14.1% | A221G (GCC→GGC) | rsmB ← | ribosomal RNA small subunit methyltransferase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 76,882 | 0 | G | C | 14.1% | 85.7 / 6.2 | 34 | A221G (GCC→GGC) | rsmB | ribosomal RNA small subunit methyltransferase B |
| Reads supporting (aligned to +/- strand): ref base G (17/12); new base C (3/2); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGGCCTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGACCCGCAG > NC_002947/76751‑77027 | cGGCCTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCGGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCcgc > 1:431372/1‑150 (MQ=255) gCCTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCgcgc < 1:368542/150‑1 (MQ=255) ccTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCa > 1:176319/1‑150 (MQ=255) cTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATTGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCAc > 2:272068/1‑150 (MQ=255) cagcagGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCcgc > 1:3937/1‑143 (MQ=255) cagcagGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCcgc < 2:3937/143‑1 (MQ=255) gCAGGTCTTGTCTCCCGTTTCGCAGCAATCATCGATCACGCTTTGGCCTGGGGCCATTTACAGCAGGTCAGCATATAGCTGCGCAGCTTCTTCCTGCACGCTCACCCAGCCTTCGCCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTc < 2:142742/150‑1 (MQ=255) cAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCg < 2:182274/150‑1 (MQ=255) ttGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGc > 1:98240/1‑150 (MQ=255) aTCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTg > 1:268901/1‑150 (MQ=255) tCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTgg > 1:418769/1‑150 (MQ=255) aGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCAt < 1:202105/150‑1 (MQ=255) ccGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCg > 1:440262/1‑150 (MQ=255) gCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCg > 2:398934/1‑150 (MQ=255) ccAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCgg > 1:363190/1‑150 (MQ=255) ccAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCgg < 2:190169/150‑1 (MQ=255) cagGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCcag < 2:418769/150‑1 (MQ=255) cagGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCcag < 1:70230/150‑1 (MQ=255) tCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGt > 2:400235/1‑150 (MQ=255) tAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTa < 1:454660/150‑1 (MQ=255) gcAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGCTGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATcg > 1:372025/1‑150 (MQ=255) cTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGc > 1:285226/1‑150 (MQ=255) cTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAg < 2:375542/129‑1 (MQ=255) cTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAg > 1:375542/1‑129 (MQ=255) aCGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGAcgg < 2:431372/150‑1 (MQ=255) cctgccAGGCTTCGCCGAAGGCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGt > 1:5741/5‑150 (MQ=255) cctgccAGGCTTCGCCGAAGGCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGt < 2:5741/146‑1 (MQ=255) tgccAGGCTTCGCCGAAGGCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTg > 2:233036/3‑150 (MQ=255) tgccAGGCTTCGCCGAAGGCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTg > 2:63895/3‑150 (MQ=255) aCCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTg > 1:421725/1‑150 (MQ=255) cccAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGa > 1:147463/1‑150 (MQ=255) cccAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGa < 2:98240/150‑1 (MQ=255) aGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGAccc < 2:268901/150‑1 (MQ=255) ttCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGACCCGCAg > 1:64608/1‑150 (MQ=255) | CGGCCTCCAGCAGGTGGCAGGTCTTGCCGCCCGGTGCGCAGCAAGCATCGAGCACGCGTTGGCCGGGGGCCAGTTCCAGCAGGTCGGCAGATAGCTGCGCAGCTTCGTCCTGCACGCTCACCCAGCCTTCGGCGAAGCCTGGCAGGCCGCGCACGTCGCAGGCTTCGGCCAGCACGATGCCATCCCGGCTGTACTGGCATGCGCTGGCGCCGATGCCGGCTTCGGCCAGCAGTGCCAGGTAGGCATCGCGGCTATGGTGACGGCGGTTGACCCGCAG > NC_002947/76751‑77027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |