| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,469,821 | A→T | 15.1% | L110Q (CTG→CAG) | algK ← | alginate biosynthesis protein AlgK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,469,821 | 0 | A | T | 15.1% | 86.2 / 9.2 | 33 | L110Q (CTG→CAG) | algK | alginate biosynthesis protein AlgK |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base T (3/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
GTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTC > NC_002947/1469673‑1469963 | gTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACcagcag > 2:210653/1‑150 (MQ=255)gTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACcagcag < 1:385568/150‑1 (MQ=255)gTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACcagcag < 1:88320/150‑1 (MQ=255) aCCTTGGGGAAACTCTGCGGGTAGCGGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTGCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg < 2:2088/150‑1 (MQ=255) ttGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCTGTGCCTGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCa > 1:361854/1‑150 (MQ=255) ggggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg < 2:55228/145‑1 (MQ=255) ggggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg > 1:55228/1‑145 (MQ=255) tGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTgg > 1:471761/1‑150 (MQ=255) cTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGc < 1:431424/150‑1 (MQ=255) tGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGCCCAA‑GCGCGCCTTGGccgcct > 2:107627/1‑144 (MQ=255) gTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGt < 2:13473/150‑1 (MQ=255) agagCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTgg < 2:182256/150‑1 (MQ=255) agCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTggcg < 2:389732/150‑1 (MQ=255) agCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTggcg > 2:339844/1‑150 (MQ=255) tttGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCtt > 2:398251/1‑150 (MQ=255) cgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgg < 1:210653/150‑1 (MQ=255) ggCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAggg > 2:133263/1‑150 (MQ=255) cGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGa < 2:471761/150‑1 (MQ=255) gCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCGGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgg < 2:372984/127‑1 (MQ=255) gCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCGGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgg > 1:372984/1‑127 (MQ=255) cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgg < 2:351431/122‑1 (MQ=255) cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAgg > 1:351431/1‑122 (MQ=255) cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc > 1:300382/1‑150 (MQ=255) aCGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAg < 1:339844/150‑1 (MQ=255) gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAggg < 1:144497/113‑1 (MQ=255) gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAggg > 2:144497/1‑113 (MQ=255) gTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCt < 1:398251/150‑1 (MQ=255) gTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCACCTCGTGTATGAGCTGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCt < 2:259483/150‑1 (MQ=255) gcgccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGAttt < 2:477086/89‑1 (MQ=37) gcgccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGAttt > 1:477086/5‑93 (MQ=37) gccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCt < 2:445341/144‑1 (MQ=255) gccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCt > 1:445341/3‑146 (MQ=255) ttGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATa > 1:252085/1‑65 (MQ=25) caccaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt > 1:435034/1‑111 (MQ=255) caccaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt < 2:435034/111‑1 (MQ=255) caccaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTc > 2:227246/1‑150 (MQ=255) | GTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTC > NC_002947/1469673‑1469963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |