| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,100,098 | G→C | 14.8% | intergenic (‑166/‑39) | PP_1875 ← / → PP_1876 | two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,100,098 | 0 | G | C | 14.8% | 67.9 / 6.1 | 27 | intergenic (‑166/‑39) | PP_1875/PP_1876 | two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base C (2/2); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
TCTGTTCATTTCAGTTGATCGCCAAGCGGGGGCCGCTTGGCGCCCCTCCGCGGGCTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATCGACTGG > NC_002947/2099964‑2100232 | tctgtTCATTTCAGTTGATCGCCAAGCGGGGGCCGCTTGGCGCCCCTCCGCGGGCTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCaaaa > 1:457430/1‑150 (MQ=255) gCCAAGCGGGGGCCGCTTGGCGCCCCTCCGCGGGCTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAgtc < 2:457430/150‑1 (MQ=255) cGCTTGGCGCCCCTCCGCGGGCTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGAcgctc > 2:358118/1‑150 (MQ=255) tccgcgggcttgcccgctcccacagggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCttt > 2:219558‑M2/89‑150 (MQ=255) CTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCCTCATGGACGCTCGCTTGATCGCTTTTCTGGAcc < 2:213849‑M1/150‑1 (MQ=255) CCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCttt > 1:192303‑M1/1‑138 (MQ=255) CCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCttt < 2:192303‑M1/138‑1 (MQ=255) CCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGcc < 1:437270‑M1/150‑1 (MQ=255) gctcccacagggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcCAAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCgcgc > 2:389722‑M2/74‑147 (MQ=255) gctcacacagggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcCAAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCgcgc < 1:389722‑M2/74‑1 (MQ=255) ccACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGAc > 1:319081/1‑114 (MQ=255) ccACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGAc < 2:319081/114‑1 (MQ=255) cacagggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCgg < 1:23310‑M2/82‑1 (MQ=255) cacagggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCgg > 2:23310‑M2/69‑150 (MQ=255) cacaGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGACGAATCgg > 1:24244/1‑150 (MQ=255) ggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcCAAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTg > 1:376591‑M2/64‑129 (MQ=255) ggcttgcgttcagcctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcCAAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTg < 2:376591‑M2/66‑1 (MQ=255) gTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTg > 1:472312/1‑150 (MQ=255) aCAGCTTAGCCCCGGGTTGAACGCAGGGGCTTTGGCAGCGGGCAGGCCCGCGTAGGGTCGCGCAGCGGCCCCCAAGAAACTGATATTTTTAGTAGTCGTCATGGGCGCTCGCTTTGTCGCTTTTCTGGACCGCGCCGAATCGGTACTGgc < 2:24244/150‑1 (MQ=255) cctggggctaagctgtacctgtgggaacgggcaagcccgcggaggggcgcGCAGCGGCTCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAAc < 1:219558‑M2/100‑1 (MQ=255) cAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAAcc > 2:298549/1‑150 (MQ=255) cTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAAc > 2:246429/1‑150 (MQ=255) tgGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGcc < 1:246429/150‑1 (MQ=255) ggcttgcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTATTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATc < 2:29563‑M2/129‑1 (MQ=255) gcaagcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGCTCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATCg > 1:14615‑M2/21‑150 (MQ=255) gcaagcccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATCg > 1:22538‑M2/21‑150 (MQ=255) cccgcggaggggcgcGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATCGACTgg < 1:358118‑M2/135‑1 (MQ=255) | TCTGTTCATTTCAGTTGATCGCCAAGCGGGGGCCGCTTGGCGCCCCTCCGCGGGCTTGCCCGTTCCCACAGGTACAGCTTAGCCCCAGGCTGAACGCAAGCCCTGTGGGAGCGGGCAAGCCCGCGGAGGGGCGCGCAGCGGCCCCCAAAAAACTGAGATTGTTAGGAGTCGTCATGGACGCTCGCTTGATCGCTTTTCTGGACCGCGCCGAATCGGTACTGGCGCGCCTTGAACCCCTGTTGCCGGCACAACGCCCGCACATCGACTGG > NC_002947/2099964‑2100232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |