Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,514,934	G→C	25.0%	P166A (CCG→GCG)	phnX ←	phosphonoacetaldehyde hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,514,934	0	G	C	25.0%	27.8 / 7.1	16	P166A (CCG→GCG)	phnX	phosphonoacetaldehyde hydrolase
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base C (2/2);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGCAGACCAGCGCCACGGTCCACATGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTCCACCACCTTGTCCATCACCACTTTCGGGTAGCCCGAGCACGAGCCGATCTTCAGGCCGTCCTGGCGTAGACCGG  >  NC_002947/2514799‑2515083                                                                                                                                        |                                                                                                                                                      ccGAGCAGACCAGCGCCACGGTCCACATGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGt                                                                                                                                         >  2:485146/1‑150 (MQ=255)                    ggTCCACATGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGc                                                                                                                      <  1:315962/150‑1 (MQ=255)                         acaTGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGCCCATggccggcccag                                                                                                                                        <  2:91239/127‑4 (MQ=255)                         acaTGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGCCCATggccggcccag                                                                                                                                        >  1:91239/1‑124 (MQ=255)                              ccGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAACCCCCAAGGCGATCACGTTGTGCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATgg                                                                                                            <  1:306038/150‑1 (MQ=255)                                ggCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTc                                                                                                          <  1:485146/150‑1 (MQ=255)                                              cAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGt                                                                                            <  1:483646/150‑1 (MQ=255)                                                               gtgtCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTcc                                                                           <  2:349261/150‑1 (MQ=255)                                                                               cGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTc                                                                                                          >  1:391307/1‑103 (MQ=255)                                                                               cGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTc                                                                                                          <  2:391307/103‑1 (MQ=255)                                                                                    gCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGCCCATggccggccc                                                                                                                                          >  2:111652/1‑65 (MQ=39)                                                                                    gCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGCCCATggccggccc                                                                                                                                          <  1:111652/66‑2 (MQ=39)                                                                                                  aaTCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTcc                                                                           <  2:372531/115‑1 (MQ=255)                                                                                                  aaTCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTcc                                                                           >  1:372531/1‑115 (MQ=255)                                                                                                                      tGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTCCACCACCTTGTCCATCACCACTTTCGGGTAGCCCGAGCACGAGCCGATCTTCAGGc                    >  2:365896/1‑150 (MQ=255)                                                                                                                           aaGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTCCACCACCTTGTCCATCACCACTTTCGGGTAGCCCGAGCACGAGCCGATCTTCAGGCCGTcc               >  2:480805/1‑150 (MQ=255)                                                                                                                                        gccATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTCCACCACCTTGTCCATCACCACTTTCGGGTAGCCCGAGCACGAGCCGATCTTCAGGCCGTCCTGGCGTAGACCgg  >  2:5726/1‑150 (MQ=255)                                                                                                                                        |                                                                                                                                                      CCGAGCAGACCAGCGCCACGGTCCACATGCCGGCACGGCGGCCTTCAAGAATGCCAGGCACGGTGTCGTCCACCTTCACGCAGGCCGCTACATCGTCAATCCCCAGCGCAATCACGTTGGCCAAGGCCTGGGCCGGCCATGGGCGGCCGTTCGGGGTTTCATCGGTGGCCACTACATGGTCGGCCACGTAGCCGTTCTGCGCAGCCAGTTCCACCACCTTGTCCATCACCACTTTCGGGTAGCCCGAGCACGAGCCGATCTTCAGGCCGTCCTGGCGTAGACCGG  >  NC_002947/2514799‑2515083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑