| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,546,869 | G→A | 40.2% | intergenic (+25/+15) | PP_2238 → / ← PP_5504 | metallopeptidase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,546,869 | 0 | G | A | 40.2% | ‑2.1 / 6.5 | 10 | intergenic (+25/+15) | PP_2238/PP_5504 | metallopeptidase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (2/2); total (4/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
TGGAGTGGCTGAACGGCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAGATGATGATCATGCTCCGGCTGGTGTAGCCCGCCGGGTTGATGCCGAACAAATAGTCACCGGCCTCTTCATCGCTATCGCCCGAGCGCGCAATG > NC_002947/2546741‑2546986 | tcgAGTGGCTGAACGGCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTa < 2:233607/148‑1 (MQ=255) cTGAACGGCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAgatgat < 1:455871/150‑1 (MQ=255) aCGGCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAGATGATGATc < 2:484952/150‑1 (MQ=255) ggCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTCGCAAACTGCCCtttt > 1:124439/1‑127 (MQ=255) ggCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTCGCAAACTGCCCtttt < 2:124439/127‑1 (MQ=255) cACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCCGTAACGCAGCGGAAAAAGGGCAGTTCGCAAACTGCCCttt < 1:250136/120‑1 (MQ=255) cACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCCGTAACGCAGCGGAAAAAGGGCAGTTCGCAAACTGCCCttt > 2:250136/1‑120 (MQ=255) aCGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTCACAGATGATGATCATGCTCCgg > 1:96805/1‑150 (MQ=255) ccTTCGTACGTGAGCGCCTGGCGCCCTTGCTCCAAGGTGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAGATGATGATCATGCTCCGGCTg < 2:96805/150‑1 (MQ=255) cGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAGATGATGATCATGCTCCGGCTGGTGTAGCCCGCCGGGTTGATGCCGAACAAATAGTCACCGGCCTCTTCATCGCTATCGCCCGAGCGCGCAATg > 1:471588/1‑150 (MQ=255) | TGGAGTGGCTGAACGGCTACCACGCCTGCGTACATGAGCGCCTGGCGCCCTTGCTCCAAGGGGATGCGCTAGCCTGGCTGGAGATGCGTACTGCACCGCTGTAACGCAGCGGAAAAAGGGCAGTTTGCGAACTGCCCTTTTCTCTATTTACAGATGATGATCATGCTCCGGCTGGTGTAGCCCGCCGGGTTGATGCCGAACAAATAGTCACCGGCCTCTTCATCGCTATCGCCCGAGCGCGCAATG > NC_002947/2546741‑2546986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |