Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	160,311	A→G	14.3%	L26L (CTT→CTC)	PP_0149 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	160,311	0	A	G	14.3%	64.1 / 4.2	28	L26L (CTT→CTC)	PP_0149	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/12);  new base G (2/2);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GCGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160162‑160457                                                                                                                                                      |                                                                                                                                                   gCGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCa                                                                                                                                                    >  1:436512/1‑150 (MQ=255)   gTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAg                                                                                                                                                  >  2:65123/1‑150 (MQ=255)   gTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAg                                                                                                                                                  >  1:459234/1‑150 (MQ=255)                           gCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGt                                                                                                                          <  2:214586/150‑1 (MQ=255)                             tGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGGTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGc                                                                                                                        >  1:425603/1‑150 (MQ=255)                                cccGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCACAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                     <  2:365693/150‑1 (MQ=255)                                            ggTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAggg                                                                                                         <  2:459234/150‑1 (MQ=255)                                             gTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGgc                                                                                                        >  1:348894/1‑150 (MQ=255)                                                cAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTGGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCa                                                                                                     <  1:403613/150‑1 (MQ=255)                                                      ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                     <  2:310186/128‑1 (MQ=255)                                                      ttGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGa                                                                                                                     >  1:310186/1‑128 (MQ=255)                                                       tGCTTGAAGCGCGGTGGCAGTTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAagc                                                                                              <  2:329449/150‑1 (MQ=255)                                                            gAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTGTAGTTCGTTGTGGGGCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCa                                                                                         >  2:66392/1‑150 (MQ=255)                                                                               cccATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTcaca                                                                      <  2:348894/150‑1 (MQ=255)                                                                                   tGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGc                                                                  <  2:165317/150‑1 (MQ=255)                                                                                          gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            <  1:313889/69‑1 (MQ=39)                                                                                          gtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTTGAGCTGCAg                                                                                                                                            >  2:313889/1‑69 (MQ=39)                                                                                                         ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagca                                                                                                                  <  1:41562/80‑1 (MQ=255)                                                                                                         ggAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGagca                                                                                                                  >  2:41562/1‑80 (MQ=255)                                                                                                                   gTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTgg                                  >  2:298650/1‑150 (MQ=255)                                                                                                                    tttGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCCCTACCTGGTCGCTGgc                                 >  2:211013/1‑150 (MQ=255)                                                                                                                                 gAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTcc                    >  2:241205/1‑150 (MQ=255)                                                                                                                                  aGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCg                   <  1:298650/150‑1 (MQ=255)                                                                                                                                      ggccgaccTGCAGCTTGAGCTGCAGGCCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCtt                      <  2:213853/137‑1 (MQ=255)                                                                                                                                      ggccgaccTGCAGCTTGAGCTGCAGGCCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCtt                      >  1:213853/7‑143 (MQ=255)                                                                                                                                      cGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTAc               <  1:66392/150‑1 (MQ=255)                                                                                                                                             cTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctcg        >  1:301082/1‑150 (MQ=255)                                                                                                                                                  gCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAg   <  2:301082/150‑1 (MQ=255)                                                                                                                                                   cTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAgg  >  1:179154/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GCGTCGGCAGGCATGTTGTCGCTCCAGCTGACCCGCACGCGGCGGTCCAGCTGTTGCTTGAAGCGCGGTGGCAGCTTGCCCATGGCCTCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGG  >  NC_002947/160162‑160457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑