| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,673,232 | G→T | 22.2% | S168I (AGC→ATC) | PP_2340 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,673,232 | 0 | G | T | 22.2% | 32.7 / 5.0 | 18 | S168I (AGC→ATC) | PP_2340 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base T (2/2); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
TATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAT > NC_002947/2673090‑2673363 | tATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAg < 2:376239/150‑1 (MQ=255) cATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCa < 1:235669/150‑1 (MQ=255) tGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGt > 2:264395/1‑150 (MQ=255) ccGGGGGTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGa > 2:388319/1‑150 (MQ=255) cccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATGTCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAcc > 2:446443/1‑150 (MQ=255) aTTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg > 1:307451/1‑150 (MQ=255) ttGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTgg < 1:388319/150‑1 (MQ=255) tGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGt < 1:396428/150‑1 (MQ=255) tGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAg > 2:238499/1‑150 (MQ=255) gagGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTgg > 2:216282/1‑125 (MQ=255) gagGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTgg < 1:216282/125‑1 (MQ=255) aCTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCgg < 1:264395/150‑1 (MQ=255) ccccAGCCTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATc > 1:72868/1‑150 (MQ=255) cccTGCGCGCTGCCCAT‑GGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGc > 1:470227/2‑150 (MQ=255) cTGCGCGCTGCCCAT‑GGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc < 1:430970/122‑1 (MQ=255) cTGCGCGCTGCCCAT‑GGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc > 2:430970/1‑122 (MQ=255) cTGCGCGCTGCCCAT‑GGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGa < 2:470227/150‑1 (MQ=255) tGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt < 2:307451/150‑1 (MQ=255) | TATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATG‑CCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAT > NC_002947/2673090‑2673363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |