| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,717,206 | G→C | 15.4% | A626A (GCG→GCC) | cti → | esterified fatty acid cis/trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,717,206 | 0 | G | C | 15.4% | 64.1 / 3.9 | 25 | A626A (GCG→GCC) | cti | esterified fatty acid cis/trans isomerase |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base C (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCTGCTGCAGCGCACCGGCAGCCTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTAC > NC_002947/2717063‑2717344 | aaGCGGCGGGAGCGCACGGGCAGCCGGAAGGCCGCACCGGACCCGATCAACCGCGGCCAGGGCGCGTTCTGCTCACGGCGGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCggg < 2:296650/150‑1 (MQ=255) tgctgcAGCGCACCGGCAGCCTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCA‑GCCCGGCCGCCGGGcgtga < 1:55944/150‑5 (MQ=255) tgcAGCGCACCGGCAGCCTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAgg < 1:135178/150‑1 (MQ=255) cTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCCGCCGGGcgt > 1:22060/1‑127 (MQ=255) cTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCCGCCGGGcgt < 2:22060/129‑3 (MQ=255) aTGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAgg < 1:409518/150‑1 (MQ=255) ccGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGt < 2:21822/150‑1 (MQ=255) ccGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAgg > 1:277390/1‑150 (MQ=255) cTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCa < 2:277390/150‑1 (MQ=255) tGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAg < 2:107854/150‑1 (MQ=255) ccAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGa > 1:26816/1‑150 (MQ=255) ttCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCGGACTGGTGTCACGCCCGGCCGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGctt > 2:243153/1‑148 (MQ=255) tCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGAATGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGgtg > 2:435997/1‑150 (MQ=255) tCACGGCCGCAGATGAGCCAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGgtg < 1:55789/150‑1 (MQ=255) ccGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGc < 1:435997/150‑1 (MQ=255) ttCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACcgcgcg > 2:313983/1‑150 (MQ=255) ttCAGCAATGCCGAGCGGTGGGTCAGCCAACTGGTTTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATACCGACATTGAAAACTCAAAACCGCTAGCGGCAGGTTTACAGCCTGCTGCGCAACcgcgcg > 2:108906/1‑150 (MQ=255) tCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCgcgcgc > 1:472679/1‑150 (MQ=255) cGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGcac > 2:188584/1‑150 (MQ=255) tGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGc > 2:10767/1‑124 (MQ=255) tGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGc < 1:10767/124‑1 (MQ=255) gTCGCTCAGCCGGCTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGtt > 2:109809/1‑150 (MQ=255) tCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTAc > 1:179564/1‑150 (MQ=255) gCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTg > 2:241891/1‑122 (MQ=255) gCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTg < 1:241891/122‑1 (MQ=255) | AAGCTGCTGCAGCGCACCGGCAGCCTGAATGCCGCACCGGACCCGATCAACCGCTGCCAGGGCGCGTTCTGCTCACGGCCGCAGATGAGCGAAGAATTCCGCAATGCCGAGCAGTCGCTCAGCCGACTGGTGTCACGCCCGGCGGCCGGGCTGAAGGTGATCAACCAGTTGCCCGAGGCGACCATGCTGCGTATCGAAGGGCAGGACGGCCAGCGTCAGGTGTACAGCCTGCTGCGCAACCGCGCGCACAGCAACGTGGCGTTCCTGCTGGGTGAGGCGTAC > NC_002947/2717063‑2717344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |