Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,845,8290TG34.6% 15.7 / 22.1 27S26R (AGT→AGG) pfeS‑IIhistidine kinase
Reads supporting (aligned to +/- strand):  ref base T (9/8);  new base G (5/4);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCA  >  NC_002947/1845686‑1845978
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gAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCCACCGCCATTCGCTGTTCTCGCAGCTGTCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATAcc                                                                                                                                                  >  2:398550/1‑150 (MQ=255)
  aCGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTg                                                                                                                                                <  1:742847/150‑1 (MQ=255)
   cGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTga                                                                                                                                               >  1:216918/1‑149 (MQ=255)
         aTCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                         <  2:216918/150‑2 (MQ=255)
             gCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGcaca                                                                                                                                     >  2:777093/1‑150 (MQ=255)
              cACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACAt                                                                                                                                    <  2:956741/150‑1 (MQ=255)
                ccGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCgatc                                                                                                                                       <  1:210840/145‑4 (MQ=255)
                ccGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCgatc                                                                                                                                       >  2:210840/1‑142 (MQ=255)
                                ttACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCtt                                                                                                                  <  2:494865/150‑1 (MQ=255)
                                       cTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGaa                                                                                                                                >  1:477234/1‑129 (MQ=255)
                                       cTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGaa                                                                                                                                <  2:477234/129‑1 (MQ=255)
                                        tcaaagacAGCGAGGCCGAGGGAACCACGGTCTACCGCCATTTTCTGTGTTTGAAGCTGGCCATCTTGCTGGTGGGCTTATGACTGCTGATGAACGACCTCAGCGATACCCGGTGCCGGCACAGGGAAACCCAGAATGCCTTCCTCTCgg                                                                                                          >  2:735728/8‑150 (MQ=255)
                                         cAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGa                                                                                                         <  2:873205/150‑1 (MQ=255)
                                                                          ccGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGTCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATgccgc                                                                        >  1:472710/1‑150 (MQ=255)
                                                                           cGCCATTCGCTGTTCTAGAAGCTGGCCATCTCGCGGGTGGGCTTCTGCCTGCTGATGATCAGCCGCAGGTGTACCTGGGGCCGTCCCATGGGAACCCAGAATGCCTACCTCTCGGAGCCGACACGCGAGACCTTGCGGGGTTATgccgc‑c                                                                      >  2:349343/1‑150 (MQ=255)
                                                                            gCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGccgc‑cg                                                                     <  1:951804/150‑1 (MQ=255)
                                                                                   gCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGa                                                              >  1:678339/1‑150 (MQ=255)
                                                                                     tGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCATAAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAAc                                                            >  2:252994/1‑150 (MQ=255)
                                                                                                      aTCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTggc                                           >  2:798527/1‑150 (MQ=255)
                                                                                                       tctcGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTggcg                                          >  1:585145/1‑150 (MQ=255)
                                                                                                            cTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                         <  1:21194/51‑2 (MQ=17)
                                                                                                            cTGGTGGGCTTCTGCCTGCTGATGATCGGCCCCAGGTATAACTGAGGCCga                                                                                                                                         >  2:21194/1‑50 (MQ=17)
                                                                                                                     ttCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTg                            <  1:798527/150‑1 (MQ=255)
                                                                                                                                gatgatCGGCCTCAGTTATACCTGGGGCCGGAACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCAGGGGCGTTCCAGC‑CACCGACCACGCGCGAAGCTTAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtgggt                 >  1:235180/1‑150 (MQ=255)
                                                                                                                                       ggCCCCAGGTATAACTGAGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtg                    <  2:1015382/140‑1 (MQ=255)
                                                                                                                                       ggCCCCAGGTATAACTGAGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAgtg                    >  1:1015382/1‑140 (MQ=255)
                                                                                                                                         ccTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCGCTGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCAt        <  1:63653/150‑1 (MQ=255)
                                                                                                                                               ttATACCTGGGGCCGGCACATGGAAACCCAGAATGc                                                                                                                     <  1:212107/36‑1 (MQ=255)
                                                                                                                                               ttATACCTGGGGCCGGCACATGGAAACCCAGAATGc                                                                                                                     >  2:212107/1‑36 (MQ=255)
                                                                                                                                               ttATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGACTGGACCAGTGGGTGGCGGCCATGCATCa  >  1:473464/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                      
GAACGGCAGATCCGCACCGTGTGGGGCAAGGGTTACGTGCTCAGTGCCAGCGAGGCGGAGTGAACCATGCTCGACCGCCATTCGCTGTTCTGGAAGCTGGCCATCTTGCTGGTGGGCTTCTGCCTGCTGATGATCGGCCTCAGTTATACCTGGGGCCGGCACATGGAAACCCAGAATGCCTTCCTCTCGGAGCCGGCACGCCAGACCTTGCGGGGTTATGCCGC‑CGAAGCTGAACAGGCCTGGCGCAGTGGCGGGCGGGCAGGGCTGGACCAGTGGGTGGCGGCCATGCATCA  >  NC_002947/1845686‑1845978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑