| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,282,817 | 0 | G | C | 36.6% | 52.0 / 45.1 | 42 | intergenic (‑166/+63) | PP_2010/PP_2011 | cytochrome b561/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/13); new base C (7/8); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCA > NC_002947/2282680‑2282955 | gcgcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGAGAAAACCGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCa < 1:892264/150‑1 (MQ=37) gcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGt < 1:750789/150‑1 (MQ=37) ctctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt < 2:727556/150‑1 (MQ=37) ctctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt > 1:727556/1‑150 (MQ=37) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCGGCCACCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGa > 1:974410/1‑150 (MQ=25) tCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTg > 2:270508/1‑150 (MQ=255) tGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGccc > 2:800021/1‑144 (MQ=255) tGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGccc < 1:800021/144‑1 (MQ=255) tGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAg < 2:966555/150‑1 (MQ=255) gCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCGGCCACCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAgag < 2:974410/150‑3 (MQ=17) gCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTgg > 2:21614/1‑128 (MQ=255) gCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTgg < 1:21614/128‑1 (MQ=255) ccGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCg < 1:270508/150‑1 (MQ=255) ggAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGa < 1:210183/150‑1 (MQ=255) aaaTGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACtgtg < 1:1005220/91‑1 (MQ=14) aaaTGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACtgtg > 2:1005220/1‑91 (MQ=14) aggtgtTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGc < 2:417149/148‑1 (MQ=255) aTGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAg > 1:3495/1‑110 (MQ=255) aTGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAg < 2:3495/110‑1 (MQ=255) ttGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 2:146844/76‑1 (MQ=255) ttGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt > 1:146844/1‑76 (MQ=255) ccGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATcacccacc > 2:101664/1‑150 (MQ=255) gCCCCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTACAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGa > 2:143642/1‑150 (MQ=17) ctctTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 1:117726/61‑1 (MQ=255) ctctTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt > 2:117726/1‑61 (MQ=255) tCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGcc > 1:672262/1‑150 (MQ=255) acaGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGc > 1:128222/1‑106 (MQ=255) acaGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGc < 2:128222/106‑1 (MQ=255) aaCTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCg > 1:295289/1‑87 (MQ=255) aaCTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCg < 2:295289/87‑1 (MQ=255) aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGt > 2:413930/1‑126 (MQ=25) aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGt < 1:413930/126‑1 (MQ=25) aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCt > 2:168614/1‑129 (MQ=25) aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCt < 1:168614/129‑1 (MQ=25) cTGCACAGTCTTGGAAGCCTGTGCAGCTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCAACGGc > 1:137166/1‑150 (MQ=255) tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGTGGGCGTGCCAGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa > 1:474144/1‑150 (MQ=37) tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGc > 2:694921/1‑87 (MQ=17) tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGc < 1:694921/87‑1 (MQ=17) tCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGtc > 1:598514/1‑94 (MQ=255) tCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGtc < 2:598514/94‑1 (MQ=255) ttGGAAGCCTGTGCAGTTGGACTGTGGGATCGGGCGTGCCCGCGAAGGGGTCg > 1:288309/1‑53 (MQ=21) ttGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGTCg < 2:288309/53‑1 (MQ=255) | GCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCA > NC_002947/2282680‑2282955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |