Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,198,9490AT29.6% 36.2 / 17.1 29intergenic (‑250/+27)ethA/PP_2806FAD‑containing monooxygenase EthA/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (9/10);  new base T (3/5);  total (12/17)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAA  >  NC_002947/3198811‑3199098
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aTTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACGGc                                                                                                                                            <  1:297353/150‑1 (MQ=255)
aTTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGc                                                                                                                                            <  1:585056/150‑1 (MQ=255)
    cAGGATGATTTGCACTTTTTACTTCGCCGATCAACGATCAGACTTGGTCAGTCTGGTAGCTATCGTCTTCGGGACGCTAGACGGGTGGGGCTGCGGTTTCTGGGGCTGTAGCGGGACTGGATTGCCGTGGTGCGTCGCTGCACGGCGatc                                                                                                                                        <  2:885277/150‑3 (MQ=255)
     aGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATc                                                                                                                                       <  1:555661/150‑1 (MQ=255)
                  ccTGTTTCTTCGCGGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCa                                                                                                                          <  2:897909/150‑1 (MQ=255)
                  ccTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCa                                                                                                                          <  2:563389/150‑1 (MQ=255)
                    tGTTACTTCGCCGATCAACGATCAGACTTGGTCAGTCTGGTAGATATCGTCATCGGAACCTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACg                                                                                                                                              <  1:622001/128‑1 (MQ=255)
                    tGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACg                                                                                                                                              <  1:621901/128‑1 (MQ=255)
                    tGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACg                                                                                                                                              >  2:622001/1‑128 (MQ=255)
                    tGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACg                                                                                                                                              >  2:621901/1‑128 (MQ=255)
                        aCTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACt                                                                                                                    <  2:227614/150‑1 (MQ=255)
                         cTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTg                                                                                                                   <  1:984837/150‑1 (MQ=255)
                           tCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCGGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGc                                                                                                                 >  1:298564/1‑150 (MQ=255)
                                gATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGccacca                                                                                                            >  1:600139/1‑150 (MQ=255)
                                        aTCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACgca                                                                                                                                                   <  2:914270/103‑1 (MQ=255)
                                        aTCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACgca                                                                                                                                                   >  1:914270/1‑103 (MQ=255)
                                                       tCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcg                                                                                     <  2:678861/150‑1 (MQ=255)
                                                         tGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcggt                                                                                   >  2:852739/1‑150 (MQ=255)
                                                                          ggAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCa                                                                  >  2:1018904/1‑150 (MQ=255)
                                                                           gAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg                                                                                                                                <  2:45537/87‑1 (MQ=255)
                                                                           gAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg                                                                                                                                >  1:45537/1‑87 (MQ=255)
                                                                                  cGAAGGGGGGCGCTGCGGCTTTCAGATCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtg                                                                                                                                    <  1:287830/76‑1 (MQ=255)
                                                                                  cGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtg                                                                                                                                    >  2:287830/1‑76 (MQ=255)
                                                                                              cTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGCCGCCGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGa                                              <  2:534394/150‑1 (MQ=255)
                                                                                                gCGGCTTTCGGCGCGGGCGGCGGCGGGGCTAGCCGTGCTGGGCCGCAGCACGGCGAATCCTGCGGGGCCGGAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGc                                            <  2:315161/150‑1 (MQ=255)
                                                                                                            gCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAg                                >  1:871669/1‑150 (MQ=255)
                                                                                                                           gaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCATTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGaa                 >  2:468458/1‑150 (MQ=255)
                                                                                                                               gCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTc                    <  1:878020/143‑1 (MQ=255)
                                                                                                                               gCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTc                    >  2:878020/1‑143 (MQ=255)
                                                                                                                                          aCGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCaa  <  1:1018904/150‑1 (MQ=255)
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ATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCAGCAA  >  NC_002947/3198811‑3199098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑