Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,762	C→T	S269L (TCG→TTG)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,762	0	C	T	100.0%	100.9 / NA	31	S269L (TCG→TTG)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

CAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAG  >  NC_002947/1851617‑1851904                                                                                                                                                  |                                                                                                                                               cAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATc                                                                                                                                            <  1:537302/150‑1 (MQ=255)                 ccAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAgc                                                                                                                            >  2:627912/1‑150 (MQ=255)                           gCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCt                                                                                                                  >  1:225829/1‑150 (MQ=255)                            cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                 >  1:157330/1‑150 (MQ=255)                                    gcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCaaa                                                                                                                       <  1:332168/136‑1 (MQ=255)                                    gcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCaaa                                                                                                                       >  2:332168/1‑136 (MQ=255)                                     cggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGc                                                                                                        <  1:1020336/150‑1 (MQ=255)                                              cgcgAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                                                               >  1:134622/1‑150 (MQ=255)                                                   gcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGACTTGCCCGCCACTGGCGTGAAGGCCGACATCAGCGGCCTGGCGAAACATACCTATTTGATCTGGCGCACGATGCAGCGCAAAGGCCTCGAGTTCAGCCAGCTCTACGAcgt                                                                                          <  2:51451/150‑1 (MQ=255)                                                                   gTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTg                                                                          >  2:189910/1‑150 (MQ=255)                                                                       tgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTgg                                                                      <  1:189910/150‑1 (MQ=255)                                                                        gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCATATCTACGACGTGCGTGCGGTGCGCGTGCTGGt                                                                     <  2:299186/150‑1 (MQ=255)                                                                        gaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGt                                                                     >  1:253970/1‑150 (MQ=255)                                                                          aCCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGc                                                                   <  2:157330/150‑1 (MQ=255)                                                                            cAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                                    <  1:666510/99‑1 (MQ=255)                                                                            cAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGc                                                                                                                    >  2:666510/1‑99 (MQ=255)                                                                              gCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                                 >  1:624970/1‑132 (MQ=255)                                                                              gCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                                 <  2:624970/132‑1 (MQ=255)                                                                                     aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                        >  2:849385/1‑150 (MQ=255)                                                                                               tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                              >  2:1001284/1‑150 (MQ=255)                                                                                                      tGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCt                                       >  1:555090/1‑150 (MQ=255)                                                                                                         cGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTggg                                    <  1:627912/150‑1 (MQ=255)                                                                                                                gCCGACATCAGCGGCCGGGCGAAACATCTCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTg                             >  1:208120/1‑150 (MQ=255)                                                                                                                  cGACATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGca                           <  1:1001284/150‑1 (MQ=255)                                                                                                                     cATCAGCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCacac                        <  2:134622/150‑1 (MQ=255)                                                                                                                          gCGGCCGGGCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtgt                   >  1:489546/1‑150 (MQ=255)                                                                                                                                  gCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                 >  1:786264/1‑48 (MQ=255)                                                                                                                                  gCGAAACATATCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                 <  2:786264/48‑1 (MQ=255)                                                                                                                                         atatCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCa    >  1:282741/1‑150 (MQ=255)                                                                                                                                          tatCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa   >  2:407893/1‑150 (MQ=255)                                                                                                                                           atCTATTTGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAg  <  2:282741/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               CAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAG  >  NC_002947/1851617‑1851904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑