| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,420,641 | Δ1 bp | 15.4% | intergenic (+81/+26) | ctpH → / ← PP_t62 | methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,420,641 | 0 | A | . | 15.4% | 66.1 / 8.9 | 26 | intergenic (+81/+26) | ctpH/PP_t62 | methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base A (13/9); new base . (2/2); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTGTT > NC_002947/2420500‑2420790 | gCAGCGCCTGCTTGGTTTGTCAGGGCAGTTGGGCGAAGGCACATCCCGCCTGAGTGCCAGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCTCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCgg > 2:178983/1‑150 (MQ=255) gCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg < 2:311356/149‑1 (MQ=255) gCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg > 1:311356/1‑149 (MQ=255) tGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACaa > 2:192290/1‑150 (MQ=255) tCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCttt < 1:341144/136‑1 (MQ=255) tCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCttt > 2:341144/1‑136 (MQ=255) ggCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACa > 1:322628/1‑150 (MQ=255) tCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGAc < 1:527551/122‑1 (MQ=255) tCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGAc > 2:527551/1‑122 (MQ=255) ccTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAAc < 1:192290/150‑1 (MQ=255) ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGaa > 1:115910/1‑145 (MQ=255) ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGaa < 2:115910/145‑1 (MQ=255) tGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTcc > 1:313585/1‑150 (MQ=255) tGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTcc > 2:325251/1‑150 (MQ=255) tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGaa < 1:325251/150‑1 (MQ=255) tGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtg > 2:2382/1‑88 (MQ=255) tGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtg < 1:2382/88‑1 (MQ=255) cAGGAGTTGTAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcgc > 1:371007/1‑150 (MQ=255) aGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGa > 1:264683/1‑104 (MQ=255) aGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGa < 2:264683/104‑1 (MQ=255) ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCtt < 2:85653/116‑1 (MQ=255) ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCtt > 1:85653/1‑116 (MQ=255) gagGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTacac < 2:322628/150‑1 (MQ=255) agGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACt > 1:513808/1‑150 (MQ=255) ttCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATccc < 2:313585/150‑1 (MQ=255) aGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGc > 1:426279/1‑150 (MQ=255) acaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGGTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttgtt < 2:365737/150‑1 (MQ=255) | GCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTGTT > NC_002947/2420500‑2420790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |