Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,783,736 C→T 13.3% R1208Q (CGG→CAG)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,783,7360CT13.3% 68.9 / 3.8 31R1208Q (CGG→CAG) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (14/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCGGT  >  NC_002947/4783590‑4783882
                                                                                                                                                  |                                                                                                                                                  
gcgcCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCGGtt                                                                                                                                                 >  1:26839/1‑150 (MQ=255)
     cATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGa                                                                                                                                            <  2:322687/150‑1 (MQ=255)
          gcgcAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATc                                                                                                                                       <  1:31013/150‑1 (MQ=255)
                     ccGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGa                                                                                                                            >  2:305989/1‑150 (MQ=255)
                      cGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGaa                                                                                                                           >  2:133876/1‑150 (MQ=255)
                         ccTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcggt                                                                                                                                        >  1:321845/1‑129 (MQ=255)
                         ccTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACGGCTGTTGCTggcggt                                                                                                                                        <  2:321845/134‑6 (MQ=255)
                                   aGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTc                                                                                                                              <  2:216999/134‑1 (MQ=255)
                                   aGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTc                                                                                                                              >  1:216999/1‑134 (MQ=255)
                                     gCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGaa                                                                                                            <  1:390307/150‑1 (MQ=255)
                                          ccGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCa                                                                                                                                         >  2:83905/1‑116 (MQ=255)
                                          ccGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCa                                                                                                                                         <  1:83905/116‑1 (MQ=255)
                                                                   gTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGc                                                                              <  1:305989/150‑1 (MQ=255)
                                                                       gCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGc                                                                          >  2:326263/1‑150 (MQ=255)
                                                                                  gTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAAt                                                                                                                       >  1:427836/1‑94 (MQ=255)
                                                                                  gTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAAt                                                                                                                       <  2:427836/94‑1 (MQ=255)
                                                                                  gTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTggc                                                               >  2:55310/1‑150 (MQ=255)
                                                                                                tGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGt                                                                                                                                                  >  1:429121/1‑53 (MQ=255)
                                                                                                tGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGt                                                                                                                                                  <  2:429121/53‑1 (MQ=255)
                                                                                                   gcCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTg                                              >  1:43684/1‑150 (MQ=255)
                                                                                                             cccATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTc                                    >  2:560697/1‑150 (MQ=255)
                                                                                                             cccATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTc                                    >  2:21788/1‑150 (MQ=255)
                                                                                                                 tCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCaga                                >  2:28864/1‑150 (MQ=255)
                                                                                                                   caccaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCaa                                           <  1:520166/137‑1 (MQ=255)
                                                                                                                   caccaccaGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCaa                                           >  2:520166/1‑137 (MQ=255)
                                                                                                                             tGATGCACCGCCAGCAACAGCCTTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGc                    <  1:28864/150‑1 (MQ=255)
                                                                                                                                     ggtcAGCAACGGCTGTTGCTGGCCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGc            <  2:464219/147‑1 (MQ=255)
                                                                                                                                     ggtcAGCAACGGCTGTTGCTGGCCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGc            >  1:464219/4‑150 (MQ=255)
                                                                                                                                             aCAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCagga                >  2:519962/1‑138 (MQ=255)
                                                                                                                                             aCAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCagga                <  1:519962/138‑1 (MQ=255)
                                                                                                                                               aGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCGgt  <  1:560697/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCGCCCATTGGCGCAGGGGTGCCGACCTGGACGGCAGGCTCACCGCCTGCCCTAGGCTAGCCTGCTGGTAGGCCGTCTGCAGGTCTTCGAACAGGATGCGCCAAGACACCCCATCCACCACCAGGTGATGCACCGCCAGCAACAGCCGTTGCTGACCATCGGCGAGTTCGAACAATGTCGCCCTGAACAGCTCGCCACGGGCCAGGTCGAGGCTGGCCTGCACCTCGTTGGCGGCGGTTTCTAGCGCTGCAAGGCTGTCCAGAGTGACTACCTGCAGGAGGGCCTGCACCGGT  >  NC_002947/4783590‑4783882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑