| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,819,447 | G→T | 9.2% | H3930N (CAT→AAT) | pvdL ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,819,447 | 0 | G | T | 9.2% | 155.4 / 3.9 | 51 | H3930N (CAT→AAT) | pvdL | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base G (23/23); new base T (3/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TGACCTCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCT > NC_002947/4819303‑4819593 | tGACCTCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGa < 2:506244/150‑1 (MQ=255)tGACCTCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGAACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGa < 1:111314/150‑1 (MQ=255) aCCTCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATg > 2:495521/1‑150 (MQ=255) tCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACa > 2:361373/1‑150 (MQ=255) cagcaTCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGAt < 2:261752/150‑1 (MQ=255) gcaTCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGAtct < 1:361373/150‑1 (MQ=255) tCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGTCGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTa > 1:32780/1‑150 (MQ=255) tCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGAGCGTTGCGATGACATTAGGCACGATCTCTa > 1:424551/1‑150 (MQ=255) tCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGGTCTCTACCTTGGCGCCGAAc > 2:460275/1‑150 (MQ=255) aGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAgc > 2:89273/1‑150 (MQ=255) gggCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAgcg > 2:448819/1‑150 (MQ=255) ggCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAgcgg < 1:3473/150‑1 (MQ=255) gCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAgcggc < 1:553419/150‑1 (MQ=255) gCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGCGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAgcggc > 2:69967/1‑150 (MQ=255) gtggtCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGc > 1:425652/1‑150 (MQ=255) tggtCGCTGGCCAGCATGCCCTGGATCAGGCACGGCACCCTCTCCAGCACGTTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGTCGCCGAACAGCGgcgccaag < 1:247538/150‑5 (MQ=255) cTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCAc < 1:423802/110‑1 (MQ=255) cTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCAc > 2:423802/1‑110 (MQ=255) gaGGGACGGCACGCTCGCCAGCACGGTGATGCCGGTGGCCTGCACGGGCGCCAGGAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGaa < 2:217284/149‑1 (MQ=255) ggACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCt > 1:189751/1‑150 (MQ=255) ggACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCt > 1:297368/1‑150 (MQ=255) aCGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCttt < 1:89273/150‑1 (MQ=255) ggCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGgcgccaagg < 2:92785/118‑6 (MQ=255) ggCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGgcgccaagg > 1:92785/1‑113 (MQ=255) cGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGGTCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAgg < 1:460275/150‑1 (MQ=255) tcCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTc < 1:448819/150‑1 (MQ=255) cAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTg < 2:338143/150‑1 (MQ=255) gCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTggg > 2:177851/1‑150 (MQ=255) aTGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCAAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACg > 1:510425/1‑150 (MQ=255) gCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATCACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTc < 1:177851/150‑1 (MQ=255) ccTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCAAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGc < 2:510425/150‑1 (MQ=255) cTGCACGTGCGCCAGCAGGCCCTGCGGGTGATGGGCGATGACATTAgg > 1:385884/1‑48 (MQ=255) cTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAgg < 2:385884/48‑1 (MQ=255) cTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGCACAGCGGCGCAGCCAGGAACTGCTAGACCGAGATGTCGCAGCTTTGCGAGGCGGTCTGGGCAATCCCGTCCTGCTCGCt > 2:271300/1‑150 (MQ=255) cTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCt > 2:372554/1‑150 (MQ=255) cgcCAGCAGGCCCTGCGGGTCATGGGCGATCACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCTAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAg < 1:69967/150‑1 (MQ=255) gcCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAgg > 1:474857/1‑150 (MQ=255) agcagGCCCTGCGGGTCATTGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCa < 2:312765/73‑1 (MQ=255) agcagGCCCTGCGGGTCATTGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCa < 2:312729/73‑1 (MQ=255) agcagGCCCTGCGGGTCATTGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCa > 1:312765/1‑73 (MQ=255) agcagGCCCTGCGGGTCATTGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCa > 1:312729/1‑73 (MQ=255) cagGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTg > 2:274537/1‑114 (MQ=255) cagGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTg < 1:274537/114‑1 (MQ=255) gCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCAc > 2:429084/1‑150 (MQ=255) cccTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCAcc < 2:297368/150‑1 (MQ=255) cTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCgaga > 2:428827/1‑83 (MQ=255) cTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCgaga < 1:428827/83‑1 (MQ=255) tGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTg < 1:495521/150‑1 (MQ=255) tCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGc > 2:144260/1‑150 (MQ=255) tCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGc > 1:394934/1‑150 (MQ=255) cATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCt < 2:102865/150‑1 (MQ=255) | TGACCTCGCCGGTCGGCAGCATCCAGCGCAGGCCGTCGAGGGCCTGGTGGTCGCTGGCCAGCATGCCCTGGATCAGGGACGGCACGCTCTCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCGCAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCT > NC_002947/4819303‑4819593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |