| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,949,986 | C→G | 13.5% | G135R (GGC→CGC) | fliM ← | flagellar biosynthesis switching/energizing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,949,986 | 0 | C | G | 13.5% | 61.0 / 3.7 | 28 | G135R (GGC→CGC) | fliM | flagellar biosynthesis switching/energizing protein |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base G (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
ATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGAC > NC_002947/4949853‑4950133 | aTGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGaaa > 1:344795/1‑150 (MQ=255) gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccac < 2:96894/150‑1 (MQ=255) gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccac < 1:40808/150‑1 (MQ=255) aGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccacca < 1:75495/150‑1 (MQ=255) ccGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAACTCGGTGGGGGTGAACTCAAGGACATCGGAATAGCAGTGGTGTGCATTGCCGCCAAAGAAATTGTCCACCAGCTTGa > 1:267909/1‑150 (MQ=255) cGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCGATGGCCGCCac < 2:340/128‑2 (MQ=255) cGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCGATGGCCGCCac > 1:340/1‑127 (MQ=255) tcaTCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCa < 2:180432/148‑1 (MQ=255) gCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGACAGTGTCCACCAGCTCGAACACCAGCTTg < 2:93821/150‑1 (MQ=255) ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAAc > 1:426753/1‑150 (MQ=255) gTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGa < 1:41773/150‑1 (MQ=255) gacgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGt < 1:250453/150‑1 (MQ=255) acTGGACCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCa > 2:21909/1‑150 (MQ=255) gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg > 1:465893/1‑150 (MQ=255) cAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCtt < 2:380709/150‑1 (MQ=255) tGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTTGAGGATGAAAAGCGAAGTGCGGCGCAGCGGCTTGATCTTGa > 2:279473/1‑150 (MQ=255) tGCGCACCACACGCAGTTCGGTCGGGATGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAACATGCAGCAGCTTGAACACCAGCTTGGCGTCGAGGCAGCCCAGCGGAGTGCCGCGCAGCGGCTTGATCTTGa > 1:433932/1‑150 (MQ=255) cacaCGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGtt > 1:112604/1‑150 (MQ=255) aGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCCGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtg > 2:549019/1‑150 (MQ=255) ttCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCAGCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggt < 2:245744/150‑1 (MQ=255) tcggtcggGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggtg < 2:547626/150‑1 (MQ=255) gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt < 2:19061/120‑1 (MQ=255) gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCa < 1:21909/150‑1 (MQ=255) gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAAAACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt > 1:19061/1‑120 (MQ=255) ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTa > 1:202117/1‑150 (MQ=255) tttggcgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg > 2:446896/3‑88 (MQ=255) tttggcgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg < 1:446896/86‑1 (MQ=255) gcCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGAc < 2:202117/150‑1 (MQ=255) | ATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGAC > NC_002947/4949853‑4950133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |