Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,949,989 C→G 14.0% D134H (GAT→CAT)  fliM ← flagellar biosynthesis switching/energizing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,949,9890CG14.0% 67.5 / 4.7 30D134H (GAT→CAT) fliMflagellar biosynthesis switching/energizing protein
Reads supporting (aligned to +/- strand):  ref base C (11/14);  new base G (2/2);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

ATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGACCTGC  >  NC_002947/4949853‑4950137
                                                                                                                                        |                                                                                                                                                    
aTGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGaaa                                                                                                                                         >  1:344795/1‑150 (MQ=255)
        gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccac                                                                                                                                 <  2:96894/150‑1 (MQ=255)
        gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccac                                                                                                                                 <  1:40808/150‑1 (MQ=255)
          aGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTccacca                                                                                                                               <  1:75495/150‑1 (MQ=255)
                ccGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAACTCGGTGGGGGTGAACTCAAGGACATCGGAATAGCAGTGGTGTGCATTGCCGCCAAAGAAATTGTCCACCAGCTTGa                                                                                                                         >  1:267909/1‑150 (MQ=255)
                 cGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCGATGGCCGCCac                                                                                                                                              >  1:340/1‑127 (MQ=255)
                 cGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCGATGGCCGCCac                                                                                                                                              <  2:340/128‑2 (MQ=255)
                      tcaTCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCa                                                                                                                   <  2:180432/148‑1 (MQ=255)
                           gCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGACAGTGTCCACCAGCTCGAACACCAGCTTg                                                                                                              <  2:93821/150‑1 (MQ=255)
                                          ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAAc                                                                                               >  1:426753/1‑150 (MQ=255)
                                               gTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGa                                                                                          <  1:41773/150‑1 (MQ=255)
                                                  gacgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGt                                                                                       <  1:250453/150‑1 (MQ=255)
                                                          acTGGACCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCa                                                                               >  2:21909/1‑150 (MQ=255)
                                                              gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg                                                                           >  1:465893/1‑150 (MQ=255)
                                                                 cAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCtt                                                                        <  2:380709/150‑1 (MQ=255)
                                                                         tGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTTGAGGATGAAAAGCGAAGTGCGGCGCAGCGGCTTGATCTTGa                                                                >  2:279473/1‑150 (MQ=255)
                                                                         tGCGCACCACACGCAGTTCGGTCGGGATGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAACATGCAGCAGCTTGAACACCAGCTTGGCGTCGAGGCAGCCCAGCGGAGTGCCGCGCAGCGGCTTGATCTTGa                                                                >  1:433932/1‑150 (MQ=255)
                                                                                cacaCGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGtt                                                         >  1:112604/1‑150 (MQ=255)
                                                                                       aGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCCGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtg                                                  >  2:549019/1‑150 (MQ=255)
                                                                                         ttCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCAGCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggt                                                <  2:245744/150‑1 (MQ=255)
                                                                                          tcggtcggGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggtg                                               <  2:547626/150‑1 (MQ=255)
                                                                                                             gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt                                                          <  2:19061/120‑1 (MQ=255)
                                                                                                             gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCa                            <  1:21909/150‑1 (MQ=255)
                                                                                                             gCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAAAACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGt                                                          >  1:19061/1‑120 (MQ=255)
                                                                                                                 ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTa                        >  1:202117/1‑150 (MQ=255)
                                                                                                                            tttggcgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg                                                                           >  2:446896/3‑88 (MQ=255)
                                                                                                                            tttggcgGCGATGGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCgg                                                                           <  1:446896/86‑1 (MQ=255)
                                                                                                                                   gcCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGAc      <  2:202117/150‑1 (MQ=255)
                                                                                                                                      aTCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGACCTg   <  2:465893/150‑1 (MQ=255)
                                                                                                                                       tCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGACCTGc  >  1:229962/1‑150 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
ATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAATTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATGACCTGC  >  NC_002947/4949853‑4950137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑