Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,070,720 G→T 9.5% P72Q (CCG→CAG)  PP_4466 ← TauD/TfdA family dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,070,7200GT 9.5% 130.7 / 2.6 42P72Q (CCG→CAG) PP_4466TauD/TfdA family dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base T (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA  >  NC_002947/5070573‑5070862
                                                                                                                                                   |                                                                                                                                              
gCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCggg                                                                                                                                              >  2:329680/1‑150 (MQ=255)
    gtggACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGaa                                                                                                                                          >  1:134944/1‑150 (MQ=255)
      ggACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATa                                                                                                                                        >  2:211661/1‑150 (MQ=255)
          gCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAg                                                                                                                                    >  2:474190/1‑150 (MQ=255)
                     cAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAg                                                                                                                         >  1:427802/1‑150 (MQ=255)
                        gTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGtga                                                                                                                      <  2:294872/150‑1 (MQ=255)
                          aTCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGtgatg                                                                                                                    <  2:499484/150‑1 (MQ=255)
                           tCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGtgatga                                                                                                                   <  1:474190/150‑1 (MQ=255)
                                 tgCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAAc                                                                                                             <  1:329680/150‑1 (MQ=255)
                                        tcgcttcgctGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGAtgt                                                                                                      <  1:542683/150‑1 (MQ=255)
                                                 tGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCACGAAGTGATGAGAGAACTTGATGTGCTGCTCAt                                                                                             >  1:65317/1‑150 (MQ=255)
                                                  gTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCTGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGa                                                                                                         <  1:442737/137‑1 (MQ=255)
                                                  gTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCTGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGa                                                                                                         <  1:442751/137‑1 (MQ=255)
                                                  gTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCTGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGa                                                                                                         >  2:442751/1‑137 (MQ=255)
                                                  gTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCTGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGa                                                                                                         >  2:442737/1‑137 (MQ=255)
                                                  gTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCACGAAGTGATGAGAGAACTTGATGTGCTGCTCATc                                                                                            <  2:65317/150‑1 (MQ=255)
                                                   ttACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCg                                                                                           <  2:130940/150‑1 (MQ=255)
                                                        cAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAAt                                                                                      <  2:534069/150‑1 (MQ=255)
                                                         aaaGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATg                                                                                     >  1:562794/1‑150 (MQ=255)
                                                          aaGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTCCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGt                                                                                    >  1:154848/1‑150 (MQ=255)
                                                           aGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTc                                                                                   <  2:134944/150‑1 (MQ=255)
                                                           aGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTc                                                                                   >  2:410093/1‑150 (MQ=255)
                                                           aGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTc                                                                                   <  2:562794/150‑1 (MQ=255)
                                                                        ttACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGtgctg                                                                                                  >  2:287058/1‑122 (MQ=255)
                                                                        ttACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGtgctg                                                                                                  <  1:287058/122‑1 (MQ=255)
                                                                              tCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTaca                                                                >  1:57079/1‑150 (MQ=255)
                                                                                          ttAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTgg                                                    >  2:8703/1‑150 (MQ=255)
                                                                                          ttAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTgg                                                    >  2:293006/1‑150 (MQ=255)
                                                                                            aGCCATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCACTTGCAGCTGTTTCGGGTGAATAACAGCTTTCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAg                                                  <  2:255773/150‑1 (MQ=255)
                                                                                                       aaaTTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTc                                       <  1:556920/150‑1 (MQ=255)
                                                                                                              tttttGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGtgatga                                                                                                                   >  2:247847/1‑67 (MQ=255)
                                                                                                              tttttGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGtgatga                                                                                                                   <  1:247847/67‑1 (MQ=255)
                                                                                                                              gcgcCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAg                  >  2:41244/1‑148 (MQ=255)
                                                                                                                              gcgcCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAg                  <  1:41244/148‑1 (MQ=255)
                                                                                                                                    tctTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTat          >  1:476327/1‑149 (MQ=255)
                                                                                                                                      ttGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTc                                       <  1:512893/119‑1 (MQ=255)
                                                                                                                                      ttGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTc                                       >  2:512893/1‑119 (MQ=255)
                                                                                                                                      ttGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGc                             <  1:80906/129‑1 (MQ=255)
                                                                                                                                      ttGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGc                             >  2:80906/1‑129 (MQ=255)
                                                                                                                                      ttGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTGGTc        >  2:234028/1‑150 (MQ=255)
                                                                                                                                            cTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGaa  <  1:393518/150‑1 (MQ=255)
                                                                                                                                               tttCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTg                                                     >  1:240753/1‑96 (MQ=255)
                                                                                                                                               tttCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTg                                                     <  2:240753/96‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCTGGTGGACGCTCGTAGAACCAGGTATCGGTGTGCCAGGTCGCTTCGCTGTTACCCAAAGCACCCGATGGCTTACCGTCTTTAGTAGTGTTAGCAATAACCAAAATTTCTTTTTGCGTTGGATGAGCGCCCTCTTGCAGCTGTTTCGGGTGAATAACAGCTTCCCCGAAGTGATGAGAGAACTTGATGTGCTGCTCATCGGTAATGTCGATATCGCGGAAACGTACAACACCGTACTGGAGCCAGATTTTTCGTACTTCGGCGACATCCTGAGCATCGTAGTCGTTGAA  >  NC_002947/5070573‑5070862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑