| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,079,177 | T→A | 9.5% | intergenic (‑374/+23) | mgtE ← / ← PP_t56 | magnesium transporter/tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,079,177 | 0 | T | A | 9.5% | 121.7 / 5.7 | 42 | intergenic (‑374/+23) | mgtE/PP_t56 | magnesium transporter/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base A (2/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
TATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAA > NC_002947/5079029‑5079317 | tATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTg > 1:506802/1‑150 (MQ=255) aTCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGa > 1:473846/1‑150 (MQ=255) nagacgaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGc > 2:313878/3‑150 (MQ=255) acgaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCttt < 2:536983/150‑1 (MQ=255) gaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCtttt > 1:517603/1‑149 (MQ=255) gaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCtttt < 2:517603/149‑1 (MQ=255) gaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCttt > 2:276461/1‑148 (MQ=255) gaTTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCttt < 1:276461/148‑1 (MQ=255) tgtgGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCNTNGGCGCANAAACAAAAAGCCNGCTCTATTGAGCGGGCTTTTTGATngg < 1:313878/150‑1 (MQ=255) gtgGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATgggg > 1:177176/1‑150 (MQ=255) aaGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGAc < 2:177176/150‑1 (MQ=255) cacaTTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTca > 1:555007/1‑150 (MQ=255) caTTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCGTGTGGATGGGGTATGGCGGACTcagc > 2:71694/1‑150 (MQ=255) tCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGTGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCg > 2:41983/1‑150 (MQ=255) cTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGtt > 2:279222/1‑150 (MQ=255) aaaCTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCg < 1:281293/111‑1 (MQ=255) aaaCTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCg > 2:281293/1‑111 (MQ=255) aaaCTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa < 1:279222/150‑1 (MQ=255) aCTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc < 2:473846/150‑1 (MQ=255) ccGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa > 2:539236/1‑150 (MQ=255) cGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGAtt > 1:395313/1‑116 (MQ=255) cGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGAtt < 2:395313/116‑1 (MQ=255) cATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATc < 2:506802/150‑1 (MQ=255) agagCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGACCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc < 2:555007/150‑1 (MQ=255) gCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt < 1:539236/150‑1 (MQ=255) aTCGACAGTCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCtttt < 2:279955/63‑1 (MQ=255) aTCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCtttt > 1:279955/1‑63 (MQ=255) aaGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATgg > 2:147871/1‑64 (MQ=255) aaGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATgg < 1:147871/64‑1 (MQ=255) aaGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt > 2:188998/1‑132 (MQ=255) aaGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt < 1:188998/132‑1 (MQ=255) tCATAGGCGCACAAACTAAAAGCCCGCTATATTGCGCGGTCTTTTTGATGGGGTATGGCGGACTCAGCAGGACTCGCACCTGGGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGAc < 2:136870/150‑1 (MQ=21) gcACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTAc > 2:428467/1‑150 (MQ=255) aaaaaGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt > 2:474201/1‑110 (MQ=255) aaaaaGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt < 1:474201/110‑1 (MQ=255) aaaaGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt < 1:133247/107‑1 (MQ=255) aaaaGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt > 2:133247/1‑107 (MQ=255) aaaGCCCGCTCTATTGAGTGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCTTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTa < 1:41983/150‑1 (MQ=255) aaaGCCCGCTCAATAGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGAt < 1:303533/119‑1 (MQ=255) aaaGCCCGCTCAATAGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCATGAt > 2:303533/1‑119 (MQ=255) cccGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAATCCGTGATTGTAGGTTTTAACCATGTTAACACTGGCTTACtta > 2:340765/1‑148 (MQ=21) ccGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGaa > 2:144308/1‑150 (MQ=255) | TATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAA > NC_002947/5079029‑5079317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |