Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,085,900	T→C	13.8%	Q36R (CAG→CGG)	PP_4476 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,085,900	0	T	C	13.8%	66.5 / 3.8	29	Q36R (CAG→CGG)	PP_4476	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/14);  new base C (2/2);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

TTCTGCCGGTTCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTGGCGTTGGAACGGATAC  >  NC_002947/5085770‑5086045                                                                                                                                   |                                                                                                                                                  ttCTGCCGGTTCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTccttgcc                                                                                                                                >  1:371113/1‑150 (MQ=255)     gCCGGTTCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCcttgccttgc                                                                                                                            <  2:35608/150‑1 (MQ=255)      ccGGTTCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCCGAATCCACACTTCCttgccttgcc                                                                                                                           >  2:270858/1‑150 (MQ=255)          ttCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCCTCCCGCTGAATCCACACTTCCTTGCCTTGCCAGAc                                                                                                                       <  1:390820/150‑1 (MQ=255)               tttCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCCGAATCCACACTTCCTTGCCTTGCCAGACCACAg                                                                                                                  >  2:82205/1‑150 (MQ=255)                        gCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATCCTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCggg                                                                                                         <  2:379714/150‑1 (MQ=255)                                  cAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGt                                                                                               >  2:327139/1‑150 (MQ=255)                                    gATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGttt                                                                                             >  1:393701/1‑150 (MQ=255)                                     aTTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCCGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTc                                                                                            <  1:270858/150‑1 (MQ=255)                                      ttGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTcc                                                                                           <  1:327139/150‑1 (MQ=255)                                                 cGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGcc                                                                                <  2:232780/150‑1 (MQ=255)                                                    gcgcAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTc                                                                             <  1:440877/150‑1 (MQ=255)                                                               aGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGa                                                                  <  1:92513/150‑1 (MQ=255)                                                               aGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCCGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCgc                                                                  <  1:82205/150‑2 (MQ=255)                                                                     cATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACCGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCGTCGAGGaccc                                                            >  2:332101/1‑147 (MQ=255)                                                                       tACTCGGTGTCGTCTTCCCCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAgg                                                          <  1:122136/150‑1 (MQ=255)                                                                          tCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTc                                                                    <  2:515612/137‑1 (MQ=255)                                                                          tCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTc                                                                    >  1:515612/1‑137 (MQ=255)                                                                                           gACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGAAGGAGGTTTCCAGCTGGGTGACGTATTCATCTTTGGTGGTCAGGCGCAAGGCGTCGGTCATTAc                                      >  2:375432/1‑150 (MQ=255)                                                                                              aTCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACaga                                   <  2:186768/150‑1 (MQ=255)                                                                                              aTCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTGGCCAGACCACAGCCCCGGCGGTGCAGGAGGTTTCCAGCTGGTTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACaga                                   <  1:268468/150‑1 (MQ=255)                                                                                                ccACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGt                                 <  2:466981/150‑1 (MQ=255)                                                                                                  aCGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGtct                               >  2:210338/1‑150 (MQ=255)                                                                                                       gATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCtt                          >  2:532248/1‑150 (MQ=255)                                                                                                              aaCTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGtt                   >  1:535988/1‑150 (MQ=255)                                                                                                               aCTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTg                  >  2:418435/1‑150 (MQ=255)                                                                                                                 tGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCCTCTTCGAGGATCAGGCGCAAGGCGTCGGCCATTACAGAGTGTCTCTTCAAGGTCgga                >  2:551217/1‑149 (MQ=255)                                                                                                                      aCGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTGGCGTTgg           <  1:418435/150‑1 (MQ=255)                                                                                                                            aTCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTGGCGTTGGAACGGa     <  1:532248/150‑1 (MQ=255)                                                                                                                               ctgctgAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTGGCGTTGGAACGGATac  >  2:296879/1‑150 (MQ=255)                                                                                                                                   |                                                                                                                                                  TTCTGCCGGTTCCATTTCCAGCTGCAGGCTGACCAGATTGGTGGCCATCGGGCGCAGCAGCAAGTTGGCATACTCGGTGTCGTCTTCCTCGACATCCACGCCGATCAGCAACTGGCCACGGCCATCCTGCTGAATCCACACTTCCTTGCCTTGCCAGACCACAGCAAAGCGGGTGCAGGAGGTTTCCAGCTGGGTGCCGTCTTCATCTTCGAGGATCAGGCGCAAGGCGTCGGTCATTACAGAGTCTCTCTTCAAGGTTGGCGTTGGAACGGATAC  >  NC_002947/5085770‑5086045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑