Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,191,879 G→A 12.1% D96N (GAC→AAC)  PP_4572 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,191,8790GA12.1% 80.6 / 3.8 33D96N (GAC→AAC) PP_4572hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/16);  new base A (2/2);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AGGTAGCCGATGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTCCTGCCCATCACCCTCAACGAAGTGC  >  NC_002947/5191733‑5192026
                                                                                                                                                  |                                                                                                                                                   
aGGTAGCCGATGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACa                                                                                                                                                  >  2:511949/1‑150 (MQ=255)
 ggTAGCCGATGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACaa                                                                                                                                                 <  1:147463/150‑1 (MQ=255)
   tAGCCGATGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTTTGGTACGACAAgg                                                                                                                                               >  2:324631/1‑150 (MQ=255)
         aTGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGANCGACCTNTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAgtgg                                                                                                                                         <  1:324523/150‑1 (MQ=255)
                 ctggcctggTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAg                                                                                                                                 <  2:470212/150‑1 (MQ=255)
                   ggcctggTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGt                                                                                                                               <  2:427749/150‑1 (MQ=255)
                    gcctagTGGAGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTg                                                                                                                              >  2:34934/1‑150 (MQ=255)
                            gcgcGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGt                                                                                                                      <  1:263739/150‑1 (MQ=255)
                                   gCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAgg                                                                                                                                >  2:80866/1‑133 (MQ=255)
                                   gCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAgg                                                                                                                                <  1:80866/133‑1 (MQ=255)
                                       aTCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGc                                                                                                           <  2:436873/150‑1 (MQ=255)
                                        tCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCt                                                                                                          >  2:403450/1‑150 (MQ=255)
                                                         aaaTCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGGACGTGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACggtg                                                                                         <  1:34934/150‑1 (MQ=255)
                                                               tCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCa                                                                                   >  1:272828/1‑150 (MQ=255)
                                                                              aGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGa                                                                    <  1:511949/150‑1 (MQ=255)
                                                                               ggTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACAACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGAc                                                                   >  2:345919/1‑150 (MQ=255)
                                                                               ggTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACAACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGAc                                                                   >  2:345956/1‑150 (MQ=255)
                                                                                gTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGAcc                                                                  <  2:162022/150‑1 (MQ=255)
                                                                                       tACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAg                                                           <  2:410909/150‑1 (MQ=255)
                                                                                           gTTTCTCGACGCCCCACTGATCGACCTCGCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTac                                                       <  1:204968/150‑1 (MQ=255)
                                                                                                 cGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAAACATCCTCGCAGACCGGCCAAGGTactgactg                                                 <  1:158728/150‑1 (MQ=255)
                                                                                                  gACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTgg                                                >  1:487121/1‑150 (MQ=255)
                                                                                                          aCTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTact                                                      <  1:428761/136‑1 (MQ=255)
                                                                                                          aCTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTact                                                      >  2:428761/1‑136 (MQ=255)
                                                                                                                  aCCTCTCGGTGAGCTGGCATTCGTTGTGGTACAACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGaaa                                <  1:345919/150‑1 (MQ=255)
                                                                                                                  aCCTCTCGGTGAGCTGGCATTCGTTGTGGTACAACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGaaa                                <  1:345956/150‑1 (MQ=255)
                                                                                                                       tcGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTc                           >  2:123911/1‑150 (MQ=255)
                                                                                                                                tGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGAAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTCCTGCCCATc                  >  2:298687/1‑150 (MQ=255)
                                                                                                                                            tgGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGACAAACTCCTGCCCATCACCCTCAACGaa      >  2:373306/1‑150 (MQ=255)
                                                                                                                                              gTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAg                                                           <  2:117801/95‑1 (MQ=255)
                                                                                                                                              gTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAg                                                           >  1:117801/1‑95 (MQ=255)
                                                                                                                                              gTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTCCTGCCCATCACCCTCAACGAAGt    >  2:323217/1‑150 (MQ=255)
                                                                                                                                                aCGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTCCTGCCCATCACCCTCAACGAAGTGc  <  1:403450/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGGTAGCCGATGTGGACCTGGCCTGGTGGCGCGGTGCCTATCAGATAAACGGGCTGAAAATCGTCAAGACCACCGGCAAGGTGCCCGTACCGTTTCTCGACGCCCCACTGATCGACCTCTCGGTGAGCTGGCATTCGTTGTGGTACGACAAGGCAGTGGTGGCGGAGGTGACCTTCGTGCACCCCGAGCTGAACTTTGTCGACGGTGGTAGCAAACAGGCGTCGCAGACCGGCCAAGGTACTGACTGGCGCCAGCAGCTGGAAAAACTCCTGCCCATCACCCTCAACGAAGTGC  >  NC_002947/5191733‑5192026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑