| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,614,099 | A→G | 13.3% | intergenic (+30/+52) | PP_4933 → / ← hldE | hypothetical protein/bifunctional heptose 7‑phosphate kinase/heptose 1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,614,099 | 0 | A | G | 13.3% | 70.3 / 3.6 | 30 | intergenic (+30/+52) | PP_4933/hldE | hypothetical protein/bifunctional heptose 7‑phosphate kinase/heptose 1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (12/14); new base G (2/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GCAGGGGTTGGTGGAAAGCAAGCCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCTTCACGGTGCCGCCATAGCCCTTGACGATATCA > NC_002947/5613964‑5614247 | gcagGGGTTGGTGGAAAGCAAGCCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCAACTGCTTGCTGGAGCGGGTAAACCCGc < 1:134956/150‑1 (MQ=255) ggTTGGTGGAAAGCAAGCCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCcag < 2:173588/150‑1 (MQ=255) caagcCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGccgccg > 2:548550/1‑150 (MQ=255) aagcCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGTGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGa > 2:53459/1‑150 (MQ=255) agcCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGaa < 2:310312/150‑1 (MQ=255) cccGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGc < 2:506977/150‑1 (MQ=255) cGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGa > 1:379544/1‑150 (MQ=255) cACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCGGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGAcc < 1:281015/150‑1 (MQ=255) aCTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGAt > 2:395990/1‑150 (MQ=255) gCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTg > 2:131099/1‑150 (MQ=255) ggCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAAt < 1:395990/150‑1 (MQ=255) aaGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTtctc < 2:338358/150‑1 (MQ=255) cgcTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGcc < 1:246726/150‑1 (MQ=255) ggAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCg > 1:101825/1‑150 (MQ=255) gAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGa < 1:131099/150‑1 (MQ=255) gcgGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGc < 1:347749/114‑1 (MQ=255) gcgGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGc > 2:347749/1‑114 (MQ=255) tCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCGTGCTGGAGCGTGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGAc < 2:497467/150‑1 (MQ=255) cTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCt < 1:21104/139‑1 (MQ=255) cTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCt > 2:21104/1‑139 (MQ=255) gCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTgg < 1:509380/129‑1 (MQ=255) gCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTgg > 2:509380/1‑129 (MQ=255) gTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCt > 1:252409/1‑150 (MQ=255) cGAAAGCTTTCACCTGCTTGCTGGGGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAAtttt > 1:119116/1‑94 (MQ=255) cGAAAGCTTTCACCTGCTTGCTGGGGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAAtttt < 2:119116/94‑1 (MQ=255) gAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCTTCACGGTgcc < 1:53459/150‑1 (MQ=255) tctgctGGGGCGGGTTTACCCGCTCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAAtt > 2:158108/3‑76 (MQ=25) tctgctGGGGCGGGTTTACCCGCTCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAAtt < 1:158108/74‑1 (MQ=25) tGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTGACATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCTTCACGGTGCCGCCATAGCCCTTGACGatat > 1:323356/1‑150 (MQ=255) gAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTGGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCTTTACGGTGCCGCCATAGCCCTTGACGATATCa > 1:120750/1‑150 (MQ=255) | GCAGGGGTTGGTGGAAAGCAAGCCCGCACGGGCGTCGCACTTGCAACGCTTTGGCGACAAGGCGCGAGCGCTGCTGGAGCGGGCGCGGTCCTCGCTGAACAAGTGACATCCCGAAAGCTTTCACCTGCTTGCTGGAGCGGGTAAACCCGCCCCAGCAGAGGGCCGCCGAAGCGACCCCAAGGTCAGATCAGTTCTTGCGAATTTTCTCGACAATCGCCGTGGTCGAGCTGTTCTCGACCAGCCCCAGCACCTTCACGGTGCCGCCATAGCCCTTGACGATATCA > NC_002947/5613964‑5614247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |