Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,732,026	A→T	12.5%	L196M (TTG→ATG) ‡	hutI ←	imidazolonepropionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,732,026	0	A	T	12.5%	86.8 / 5.8	32	L196M (TTG→ATG) ‡	hutI	imidazolonepropionase
Reads supporting (aligned to +/- strand):  ref base A (14/14);  new base T (2/2);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

ATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCAT  >  NC_002947/5731888‑5732170                                                                                                                                           |                                                                                                                                                 aTGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCTTGGCCGGcgaag                                                                                                                                       <  1:404205/150‑5 (MQ=255)     cAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACAtctc                                                                                                                                   <  1:92050/150‑1 (MQ=255)          gttCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTcac                                                                                                                              <  2:48984/150‑1 (MQ=255)               gCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGAtg                                                                                                                         >  2:355243/1‑150 (MQ=255)                 aTGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCTCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGAtgtg                                                                                                                       >  2:347043/1‑150 (MQ=255)                      gCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGa                                                                                                                  >  1:412944/1‑150 (MQ=255)                       cTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGAt                                                                                                                 <  2:561617/150‑1 (MQ=255)                                   gCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGc                                                                                                     <  2:512621/150‑1 (MQ=255)                                     ccAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCATGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGccc                                                                                                   >  1:501617/1‑150 (MQ=255)                                       aGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGa                                                                                                 <  1:186185/150‑1 (MQ=255)                                          tCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGAccc                                                                                              <  2:412944/150‑1 (MQ=255)                                                   ttGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACCCGAGATGTTCGCCGTAGGTAACCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTcc                                                                                     >  1:154274/1‑150 (MQ=255)                                                        gaACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCggggg                                                                                <  1:355243/150‑1 (MQ=255)                                                              ccGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGc                                                                          <  1:347043/150‑1 (MQ=255)                                                                          ggCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAg                                                              <  1:279785/150‑1 (MQ=255)                                                                                 gagaACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTg                                                       <  2:456357/150‑1 (MQ=255)                                                                                  agaACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTgg                                                      <  2:154274/150‑1 (MQ=255)                                                                                      cgcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGgcacg                                                  >  1:11617/1‑150 (MQ=255)                                                                                       gcgAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGgcccgc                                                 >  1:193050/1‑150 (MQ=255)                                                                                               ttCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCgg                                                                                   <  2:115301/108‑1 (MQ=255)                                                                                               ttCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCgg                                                                                   >  1:115301/1‑108 (MQ=255)                                                                                                    aGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCa                                    >  2:295018/1‑150 (MQ=255)                                                                                                      aaGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGc                                  >  1:108831/1‑150 (MQ=255)                                                                                                           atccactgcatccaccAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCtcgtt                             >  2:195982/1‑149 (MQ=255)                                                                                                                 cgcatccaccAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAg                       >  1:477187/1‑150 (MQ=255)                                                                                                                   catccaccAGGCCTTCGCCGGCCAAGGCCGGGAACATCTCGTCACAGATGTGCGCGATGTGGTCGTCGGACCGACCCGCGTACGCCGGGGGCGAAGCGTGCGCCGCCAGGCACGTGGAACGCACCGTCAGCGGCAGCTCGTCGGCCAggc                     >  2:84741/1‑150 (MQ=255)                                                                                                                           aGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTGACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGcc             <  2:28970/150‑1 (MQ=255)                                                                                                                               tgttgCCGGCCTTGGCCGGCGACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTc         >  1:362282/5‑150 (MQ=255)                                                                                                                                 ttgCCGGCCTTGGCCGGCGACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCa                                                               >  2:445827/3‑94 (MQ=255)                                                                                                                                 ttgCCGGCCTTGGCCGGCGACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCa                                                               <  1:445827/92‑1 (MQ=255)                                                                                                                                     cGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCgcagca   >  1:547244/1‑150 (MQ=255)                                                                                                                                      ggCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCAt  <  1:295018/150‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                 ATGACAGTTGTTCGGCATGCAGCTTGACCGGCAGGCCCAGTTCGCGCGCCTTGATGAACAGCCGCTCGACCTGGGCCGGCGAGAACGCGAGGTGTTCGCAGAAGGCATCCACCGCATCCACCAGGCCTTCGCCGGCCAAGGCCGGCAACATCTCGTCACAGATGTGCGCGATGTAGTCGTCGGCCCGACCCGCGTACTCCGGGGGCAAAGCGTGCGCCGCCAGGCACGTGGCACGCACCGTCAGCGGCAGCTCGTCGGCCAGGCGGCGGGCCACTCGCAGCAT  >  NC_002947/5731888‑5732170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑