| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,079,777 | C→A | 12.1% | G45G (GGC→GGA) | PP_5331 → | acyl‑CoA thioesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,079,777 | 0 | C | A | 12.1% | 88.3 / 3.0 | 33 | G45G (GGC→GGA) | PP_5331 | acyl‑CoA thioesterase |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01 | |||||||||||
GAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAGATGATGG > NC_002947/6079630‑6079925 | gagGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCAc > 2:252431/1‑150 (MQ=255) cccATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTTCGCAGATGGACCTTGCAGGCACGGccg > 1:257462/1‑149 (MQ=255) ccATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCAt < 1:271280/150‑1 (MQ=255) cATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATg < 2:80799/150‑1 (MQ=255) cATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATg < 1:539808/150‑1 (MQ=255) aTCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATgg < 2:418208/150‑1 (MQ=255) gCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGc < 1:80696/150‑1 (MQ=255) gAACAAGAAGATCCAATCACGCAAGTCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcggc > 2:333057/1‑150 (MQ=255) cAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATCTTTTCGGCGGTTTGCGGTTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGcc < 1:351254/150‑1 (MQ=255) gACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCGTGCTTCCGCGTAGCCACCGTCGCCATCGAc < 1:298625/150‑1 (MQ=255) gACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGAc < 1:413195/150‑1 (MQ=255) aCCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGAcc < 2:82435/150‑1 (MQ=255) aCCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGAcc > 1:464993/1‑150 (MQ=255) cTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGc > 1:557248/1‑150 (MQ=255) aTCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATg > 1:242867/1‑141 (MQ=255) aTCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATg < 2:242867/141‑1 (MQ=255) ccACCGCCTTGCTGCGAGAATCCAACGGGTTTGGCGCGCTTTTCGGCTGTTGGCTGGTTGCGCAGATGGTCCTGGCAGGCTCGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTgg < 2:257462/149‑1 (MQ=255) aCCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTa > 1:471945/1‑150 (MQ=255) cATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCg > 2:153589/1‑150 (MQ=255) gagaAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACGTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCg > 2:365157/1‑150 (MQ=255) aaCGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAg < 2:557248/150‑1 (MQ=255) gTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGAACGGCCATGGCCa > 1:392558/1‑65 (MQ=255) gTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGAACGGCCATGGCCa > 1:392415/1‑65 (MQ=255) gTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGAACGGCCATGGCCa < 2:392415/65‑1 (MQ=255) gTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGAACGGCCATGGCCa < 2:392558/65‑1 (MQ=255) tttGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCt < 1:375246/150‑1 (MQ=255) gCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCt > 1:282723/1‑150 (MQ=255) cGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCta < 2:464993/150‑1 (MQ=255) tttCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCa > 2:183611/1‑150 (MQ=255) aTGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCg > 1:498054/1‑150 (MQ=255) ggACCTGGCAGTCACGGCCATGGCCAGCAGTGTCGCCGGCGGCGGCGTAGCCACCGTCGCCATCGACCGCATGCTCTTTCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGAt < 1:549135/150‑1 (MQ=255) aCCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATcc > 1:324117/1‑150 (MQ=255) cTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAg > 1:281458/1‑150 (MQ=255) aggcaCGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAgatgat > 1:528506/1‑150 (MQ=255) aggcaCGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAgatgat > 1:238346/1‑150 (MQ=255) gcaCGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAGATGATgg > 2:506840/1‑150 (MQ=255) | GAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACCGCATGGCCTTCCTGGTACCCGTCGCGGTCGGCGCACAGCTGTCTTTCTATACCCAGACCCTGGAAATCGGCCGCAGCTCGATCCAGATGATGG > NC_002947/6079630‑6079925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |