| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,129,039 | G→T | 15.4% | G60* (GGA→TGA) | PP_5376 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,129,039 | 0 | G | T | 15.4% | 61.6 / 3.5 | 26 | G60* (GGA→TGA) | PP_5376 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/14); new base T (2/2); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
TGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGC > NC_002947/6128893‑6129185 | tGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGaa < 2:509868/150‑1 (MQ=255) aTGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTa < 1:370513/150‑1 (MQ=255) ttCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGt < 2:191000/150‑1 (MQ=255) tCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGtt < 1:295331/150‑1 (MQ=255) tCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGtt < 2:522510/150‑1 (MQ=255) cgATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCAGTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGa < 2:89703/150‑1 (MQ=255) tGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtgt < 2:506530/150‑1 (MQ=255) aGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGAc < 1:299890/150‑1 (MQ=255) gATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGa > 1:505640/1‑150 (MQ=255) gCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCtg < 1:275818/150‑1 (MQ=255) cGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGa > 2:452358/1‑150 (MQ=255) aaCAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCt > 2:94217/1‑150 (MQ=255) gCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGaa > 1:27081/1‑150 (MQ=255) aTGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTg < 1:390420/91‑1 (MQ=255) aTGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTg > 2:390420/1‑91 (MQ=255) gcgcgcATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGCCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTc < 2:160200/150‑1 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg > 2:130852/1‑139 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg < 1:130844/139‑1 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg > 2:130844/1‑139 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg < 1:130852/139‑1 (MQ=255) gAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCt < 1:452358/150‑1 (MQ=255) ttGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGGCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGAGCAGCTGGCa > 1:68633/1‑150 (MQ=255) cACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAAt < 2:505640/150‑1 (MQ=255) cTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGa < 2:414353/96‑1 (MQ=255) cTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGa > 1:414353/1‑96 (MQ=255) tACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGc > 2:313233/1‑150 (MQ=255) | TGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGC > NC_002947/6128893‑6129185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |