| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,129,123 | G→T | 15.4% | E88* (GAA→TAA) | PP_5376 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,129,123 | 0 | G | T | 15.4% | 65.3 / 3.5 | 26 | E88* (GAA→TAA) | PP_5376 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/10); new base T (2/2); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTG > NC_002947/6128976‑6129271 | gCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGaa > 1:27081/1‑150 (MQ=255) gcgcgcATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGCCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTc < 2:160200/150‑1 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg < 1:130852/139‑1 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg > 2:130852/1‑139 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg > 2:130844/1‑139 (MQ=255) tCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACTGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATTAAGACTCGACAg < 1:130844/139‑1 (MQ=255) gAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCt < 1:452358/150‑1 (MQ=255) ttGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGGCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGAGCAGCTGGCa > 1:68633/1‑150 (MQ=255) cACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAAt < 2:505640/150‑1 (MQ=255) cTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGa > 1:414353/1‑96 (MQ=255) cTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGa < 2:414353/96‑1 (MQ=255) tACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGc > 2:313233/1‑150 (MQ=255) tGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAAtt < 1:189376/150‑1 (MQ=255) gATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTgg < 1:270428/119‑1 (MQ=255) gATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTgg > 2:270428/1‑119 (MQ=255) aaGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGGTACCGAAAGGGAGCTCTTAAGGCTGTcc > 2:5031/1‑150 (MQ=255) tGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCaaa > 1:292349/1‑150 (MQ=255) cTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGaaaa < 2:27081/150‑1 (MQ=255) cTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGcc > 2:282417/1‑150 (MQ=255) cGTGATGCCTGCGCAAATGACCTATGAACACTCGACAGGCAACATC‑TCATCGCGCTAATACCTCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg < 2:158391/150‑1 (MQ=255) tGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACgg > 2:226079/1‑150 (MQ=255) gcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTgg > 2:41119/1‑150 (MQ=255) aTGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCa < 1:41119/150‑1 (MQ=255) tGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGTCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAg > 1:262989/1‑150 (MQ=255) cTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGc > 1:551827/1‑150 (MQ=255) tGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATCCCGTGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTg < 1:5031/150‑1 (MQ=255) | GCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATCCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACAT‑TCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTG > NC_002947/6128976‑6129271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |