| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 327,553 | T→C | 10.0% | Y61C (TAC→TGC) | PP_0269 ← | glutamate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 327,553 | 0 | T | C | 10.0% | 105.9 / 2.6 | 40 | Y61C (TAC→TGC) | PP_0269 | glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (17/19); new base C (2/2); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GGCGCTTTCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTGAAGACAAGGCA > NC_002947/327408‑327692 | ggCGCTTTCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGtt > 2:306203/1‑150 (MQ=255) cgcTTTCGTTCTTGGCGTTCACATCTACCAACGAGTGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGCCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGc < 1:405564/150‑1 (MQ=255) tttCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGTATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTc < 2:69300/150‑1 (MQ=255) tttCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTc > 1:396244/1‑150 (MQ=255) ttCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCg > 1:27414/1‑150 (MQ=255) cGTAGACCAACCAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCttt < 1:24165/150‑1 (MQ=255) aGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGgc < 2:414466/150‑1 (MQ=255) gcgTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCAtt < 2:189243/150‑1 (MQ=255) tGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAga < 2:260966/150‑1 (MQ=255) gCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGAATTGGGATAGGTCATTTAgagcgac > 2:315039/1‑150 (MQ=39) aaCGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTg < 1:306203/150‑1 (MQ=255) cGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGag > 1:185160/1‑150 (MQ=255) aGCTTGTCGTCGTCACATTAGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGAACCGCATGGTTGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGagcagc > 2:430342/1‑150 (MQ=255) tGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCgg > 1:527741/1‑150 (MQ=255) gTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAg > 2:166420/1‑150 (MQ=255) tGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAg > 1:542556/1‑150 (MQ=255) tGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAg < 1:464397/150‑1 (MQ=255) tGGCGGACTTCCGGGCTGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCAGGGTGACTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTAACGGCCACAGCCAg < 2:26554/150‑1 (MQ=255) tttCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGtt > 2:1390/1‑150 (MQ=255) ttCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTg < 2:27414/150‑1 (MQ=255) gATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTa < 1:33927/42‑1 (MQ=255) gATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTa > 2:33927/1‑42 (MQ=255) aTGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAgg > 1:542154/1‑150 (MQ=255) aTGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAgg > 1:395978/1‑150 (MQ=255) tttCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCa < 1:166420/150‑1 (MQ=255) tCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTgg < 2:527741/150‑1 (MQ=255) tCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTgg > 1:324105/1‑150 (MQ=255) ggTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTgg < 2:17068/128‑1 (MQ=255) ggTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTgg > 1:17068/1‑128 (MQ=255) aCGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGt > 1:424995/1‑150 (MQ=255) cGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTg < 2:396244/150‑1 (MQ=255) cccAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTGAAGACAAgg < 1:1390/150‑1 (MQ=255) cccAGGATCGGGTAGTTGCGTCCCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTGAAGACAAgg < 1:404421/150‑1 (MQ=255) cccAGGATCGGGCAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTAcc > 2:147390/1‑93 (MQ=255) cccAGGATCGGGCAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTAcc < 1:147390/93‑1 (MQ=255) cccAGGATCGGGCAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTAcc > 2:147145/1‑93 (MQ=255) cccAGGATCGGGCAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTAcc < 1:147145/93‑1 (MQ=255) cAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTGAAGACAAGGCa < 2:424995/150‑1 (MQ=255) ggATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGaaa < 1:177502/134‑1 (MQ=255) ggATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGaaa > 2:177502/1‑134 (MQ=255) | GGCGCTTTCGTTCTTGGCGCGGGCGTAGACCAGCGAGCGTTGCGAGCGGGAGAACGGCAGCTTGTCGTCGTCACCTTCGATCAGGTACTGGCGGATTTCCGGGCGGATGGTTTCGATCAGGTAACGGATATTGCCCAGGATCGGGTAGTTGCGTCGCACCGCATGGTGGCTTTGGCGCAGGTCATTCAGACCGACCAGGCTGAGCAGCGCGGTGATCAGCGTTACCGGCCACAGCCAGGCATGTTGGGCCAGCAGGGGCAGGCTGGCGAAAGTGAAGACAAGGCA > NC_002947/327408‑327692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |