| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 655,911 | G→T | 19.9% | S145R (AGC→AGA) | PP_0563 ← | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 655,911 | 0 | G | T | 19.9% | 38.4 / 2.2 | 20 | S145R (AGC→AGA) | PP_0563 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (7/9); new base T (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGAGCGGCCAGCA > NC_002947/655768‑656056 | tcggtcAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGaa < 1:360354/150‑1 (MQ=255) gCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAg > 2:555147/1‑150 (MQ=255) tGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGTCTGGAAGCTTCCAgcc < 1:413813/150‑3 (MQ=255) tGCGGGTGAGCACGCCGGCGCCGGCCAGTACACGCTCGGTATACATGGCCTGTGTGCGAGAATCGTCGATTATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCATGCTGGAAGCTTCCAGAc < 1:238157/150‑1 (MQ=255) ggTGAGCATGCCGGCGCGGTCCAGTACAAGCTCGGTGTACATGGCCTGTGTGCGCTAATCGTCGATGATCAGGACGCGCCAAGGAGCGTGAGGGGCGGTGTTGGGCAGTTGTTCGACCGTTTCAAGGAGGCTAACAGCTTCCAGACTGcc > 2:240384/1‑150 (MQ=255) gTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCACCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCg < 2:409441/150‑1 (MQ=255) aTGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGTCTGGAAGCTTCCAGCCTGctt > 1:365328/1‑142 (MQ=255) aTGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGTCTGGAAGCTTCCAGCCTGctt < 2:365328/144‑3 (MQ=255) ccggcCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGa < 1:106423/150‑1 (MQ=255) aGTACACGCTCGGTGTACCTGGCCTGGGTCCGCGAATCGGCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTCGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGAccgccg < 1:266335/150‑1 (MQ=255) gCCTGTGTGCGCGCATCGTCGATTATCAGCACGCGCAGCGGGTCGTGAGGGGCGGGGTTGGTCCGCAGTTCGACCTTTTCCAGCAGGCTGGCAGCTTCCAGACTGCCGGTGAAAAAGTCCTGAACGCCGGCGCGCACTGCGGCAAGGCgg > 2:246308/1‑150 (MQ=255) cccTGTTTGCCCGAATCGTCGATGATACGCAAGCGCAACGCGTCCTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGGGCACTGCGGCAAGGCgg < 2:234905/149‑1 (MQ=255) atgatCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGa < 1:555147/150‑1 (MQ=255) gCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTga > 2:380706/1‑150 (MQ=255) cACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGCGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTgaa > 2:534066/1‑150 (MQ=255) aCCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGa > 2:239411/1‑150 (MQ=255) aCCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGa < 1:153261/150‑1 (MQ=255) ttttCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGAGCg > 1:179492/1‑150 (MQ=255) gcagTCTGGAAGCTTCCAGCCTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGAGCGGCcagca > 2:452248/1‑150 (MQ=255) gcagGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGAGCGGCcagca < 2:179492/150‑1 (MQ=255) | TCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGCGCGAATCGTCGATGATCAGCACGCGCAACGGGTCGTGAGGGGCGGTGTTGGTCAGCAGTTCGACCTTTTCCAGCAGGCTGGAAGCTTCCAGACTGCCGGTGAGAAAGTCCTGACCGCCGGCGCGCACTGCGGCAAGGCGGGTAGGGGTGTCGGCTTCGTGATGGCTGAAGAACAGCAACGGAATGGAGCGTGCCAGGCCCTGCTGCACCTGAGCGGCCAGCA > NC_002947/655768‑656056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |