| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 699,725 | A→G | 46.9% | intergenic (+115/‑129) | PP_t06 → / → PP_23SD | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 699,725 | 0 | A | G | 46.9% | ‑0.7 / 39.8 | 33 | intergenic (+115/‑129) | PP_t06/PP_23SD | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (7/10); new base G (7/8); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01 | |||||||||||
GCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAA > NC_002947/699584‑699802 | gCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAacac < 1:279121/150‑1 (MQ=255)gCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTAATTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAacac < 2:438759/150‑1 (MQ=255) cGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGttt < 1:277103/150‑1 (MQ=38) ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑‑AACACCAGTTTCACTGCtggt < 1:141659/150‑1 (MQ=255) gCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCtggtgg > 1:11403/1‑150 (MQ=39) cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGa > 2:482717/1‑150 (MQ=39) caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACAAGTTTAACTGATGGTGGGTCa > 1:218143/1‑150 (MQ=255) ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAgg > 2:54648/1‑150 (MQ=255) ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAgg > 2:480361/1‑150 (MQ=255) tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTaa < 2:545231/150‑1 (MQ=38) ttCGACGTACGCAGTTACTTGTCAGAACTTAGAAATGAACAGTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg < 2:542881/150‑1 (MQ=255) gCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTa > 2:342847/1‑150 (MQ=39) cAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAACAATTCGGCTCTGTG‑TAGAAGTGCCGTGGGAGTTCATGTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 1:294537/150‑1 (MQ=14) cAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 1:54648/150‑1 (MQ=255) cAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 1:480361/150‑1 (MQ=255) cAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 2:549645/150‑1 (MQ=38) aGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCa < 1:332958/111‑1 (MQ=255) aGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCa > 2:332958/1‑111 (MQ=255) gTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa < 1:301837/150‑1 (MQ=38) ttGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt < 1:342847/150‑1 (MQ=35) ttGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt < 2:351680/150‑1 (MQ=35) cAGAACTTAGAACTAAACATTCAGCTTGTAAGTTGATTGCTGTCTTTTGTCAGATCGTTCTTTAAAAGGCGGGGTATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTc < 1:239838/150‑1 (MQ=11) cAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTc > 1:204891/1‑150 (MQ=25) ttAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCtggt < 2:393925/109‑1 (MQ=255) ttAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCtggt > 1:393925/1‑109 (MQ=255) aGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGc < 2:293952/150‑1 (MQ=35) aaTGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAAc < 1:546998/150‑1 (MQ=34) aTGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT‑GAACACCAGTTTCACTGCTGGTGGATCAGAGAAAGTTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACg > 2:45433/1‑150 (MQ=11) gAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa > 1:479450/1‑150 (MQ=35) aaCATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAtt > 1:248348/1‑121 (MQ=37) aaCATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAtt < 2:248348/121‑1 (MQ=37) aTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGaa > 1:185433/1‑150 (MQ=35) aTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATGTGGATATGTGATAGATATAGGCT‑GAACAACAGTTTCACTGCTGGTGGCTCAGGCTAAGGTAAAATTTGTGAGGTCTGCTCGAAAGAGCAACGTACGaa > 2:33107/1‑150 (MQ=11) | GCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT‑GAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAA > NC_002947/699584‑699802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |