| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 947,116 | C→A | 22.2% | E86* (GAA→TAA) | norR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 947,116 | 0 | C | A | 22.2% | 34.7 / 4.8 | 18 | E86* (GAA→TAA) | norR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (10/4); new base A (2/2); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
GGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCAGCCATTCCCCGTCCAGTCGCAGCAGTGCTGCAGCATCGCACGGCAGC > NC_002947/946967‑947261 | ggCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTc < 1:244525/150‑1 (MQ=255) ccATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTg > 2:519745/1‑150 (MQ=255) gACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTc < 2:66969/150‑1 (MQ=255) cATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGAGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTACGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCt > 2:266765/1‑150 (MQ=255) aaCGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGAGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTACGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGAcc < 1:266765/150‑1 (MQ=255) ccATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCgcc > 1:372423/1‑150 (MQ=255) cTTCAAGGTCGGCATCGGGGGCATTGACCGAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCgg > 1:302034/1‑150 (MQ=255) ttCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCggg > 1:159406/1‑150 (MQ=255) cATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGa > 2:132534/1‑129 (MQ=255) cATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGa < 1:132534/129‑1 (MQ=255) cGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCa < 1:561321/150‑1 (MQ=255) ggCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGc < 1:519745/150‑1 (MQ=255) tGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCg > 1:293226/1‑150 (MQ=255) aaCCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCa > 1:373764/1‑150 (MQ=255) gggCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTACGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGcc < 2:210783/114‑1 (MQ=255) gggCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTACGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGcc > 1:210783/1‑114 (MQ=255) cAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCAGCCATTCCCCGTCCAGTCg > 2:212365/1‑150 (MQ=255) tGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCAGCCATTCCCCGTCCAGTCGCAGCAGTGCTgcag > 2:564522/1‑150 (MQ=255) ggtTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCAGCCATTCCCCGTCCAGTCGCAGCAGTGCTGCAGCATCGCACGgcagc > 2:524944/1‑150 (MQ=255) | GGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAACCATCGTAGGGGTCGGGCAGTTCGGAATCGCTGGCGAATCGGGTGGGTTCCGGGCGGCTGAGCAGTATCTGGAAACGCGGGTGCTCGCTGACCCGAAAGCGCCGGCCGAGGGTGTCGGGGCTAAGGCCATCCACTGCCAGCGGCACCAGCCATTCCCCGTCCAGTCGCAGCAGTGCTGCAGCATCGCACGGCAGC > NC_002947/946967‑947261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |