| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 162,281 | A→T | 8.5% | intergenic (‑315/+59) | PP_0153 ← / ← scpC | hypothetical protein/propionyl‑CoA:succinate CoA transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 162,281 | 0 | A | T | 8.5% | 130.1 / 4.3 | 47 | intergenic (‑315/+59) | PP_0153/scpC | hypothetical protein/propionyl‑CoA:succinate CoA transferase |
| Reads supporting (aligned to +/- strand): ref base A (24/19); new base T (2/2); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
CGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACG > NC_002947/162133‑162426 | cGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCaa < 1:312771/150‑1 (MQ=255) ttATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCcg > 1:384877/1‑150 (MQ=255) tATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCcgc < 1:530900/150‑1 (MQ=255) tGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTCCGGCCTCTTCGCGGGCTTGCCCGCGaatggg < 2:384877/150‑5 (MQ=38) cGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGcc < 1:198511/150‑1 (MQ=255) cGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGcc < 1:462697/150‑1 (MQ=255) tttGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAG‑GGCCGGaa < 2:310608/150‑1 (MQ=255) aagaagGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGaa > 2:511221/1‑150 (MQ=255) agGTGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCTTGCCCGCGaatgg < 1:29045/131‑4 (MQ=255) agGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCTTGCCCGCGaatgg > 2:29045/1‑128 (MQ=255) gggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAggg < 1:133796/150‑1 (MQ=255) ggATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGgc > 2:484231/1‑150 (MQ=255) tCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGACACCGtt > 1:432070/1‑150 (MQ=255) gggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGTTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAc < 1:68179/150‑1 (MQ=255) gggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGGCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAc > 2:157326/1‑150 (MQ=255) gggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAc > 1:511739/1‑150 (MQ=255) ggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAcc > 1:368996/1‑150 (MQ=255) tCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATcc < 2:368996/150‑1 (MQ=255) tCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATcc < 2:511739/150‑1 (MQ=255) aGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGc > 1:419135/1‑150 (MQ=255) gCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGcc > 2:162502/1‑150 (MQ=255) taCAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATgcgc > 1:561082/1‑150 (MQ=255) taCAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGCGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATgcgc > 2:407746/1‑150 (MQ=255) cAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGccc > 1:529853/1‑150 (MQ=255) aTTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGTCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGt < 1:344554/150‑1 (MQ=255) ttATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGtt < 2:117762/150‑1 (MQ=255) tgctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGACGGGCCACCGATCCGTATCACATACATGTCCGCGGGCCAGCGTGCGCCCGATTTCTTcc > 2:93405/1‑150 (MQ=255) ctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAg < 2:529853/150‑1 (MQ=255) gtagAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGtt > 2:228650/1‑150 (MQ=255) gtagAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGGTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCCTGCGCCCGGTTTCTTCCAGGtt > 2:55115/1‑150 (MQ=255) gagGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTc < 1:228650/150‑1 (MQ=255) gggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTg < 2:561082/150‑1 (MQ=255) ggTGTCGTAGTGATAGAAGGGTTTGACTGTTAGGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGc < 2:563992/150‑1 (MQ=255) ggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGc > 1:345013/1‑150 (MQ=255) tGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCa > 1:265317/1‑150 (MQ=255) tGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCa < 1:162502/150‑1 (MQ=255) tGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCa < 2:491218/150‑1 (MQ=255) cGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGgc < 2:494438/79‑1 (MQ=255) cGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGgc > 1:494438/1‑79 (MQ=255) gTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTc > 2:356357/1‑150 (MQ=255) tttGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGccc > 2:188479/1‑107 (MQ=255) tttGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGccc < 1:188479/107‑1 (MQ=255) tttGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAgg > 2:209164/1‑150 (MQ=255) tGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGAATCAGCTGGCCGGCATGCGCCCGGTTTCTTCCAGGATTTCGTGCCAGCTCAGGCGTTTGCGCAGGAcgtgg > 1:439569/1‑149 (MQ=255) gCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGAt > 1:243643/1‑74 (MQ=255) gCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGAt < 2:243643/74‑1 (MQ=255) cccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATg > 2:248182/1‑150 (MQ=255) gggCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACg > 1:344134/1‑150 (MQ=255) | CGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACG > NC_002947/162133‑162426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |