Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,693,832 C→A 14.2% R87S (CGC→AGC)  PP_1489 → CheW domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,693,8320CA14.2% 73.6 / 2.3 28R87S (CGC→AGC) PP_1489CheW domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (13/11);  new base A (2/2);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTGCGCCAGGA  >  NC_002947/1693708‑1693980
                                                                                                                            |                                                                                                                                                    
cAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAgg                                                                                                                             <  2:504422/150‑1 (MQ=255)
         gacccAGGCAGGTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCATCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCa                                                                                                                    >  2:313624/3‑150 (MQ=255)
          gCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAg                                                                                                                   >  2:358141/1‑150 (MQ=255)
            ccAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCt                                                                                                                 >  2:108895/1‑150 (MQ=255)
              aGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTg                                                                                                               <  1:108895/150‑1 (MQ=255)
                  aCCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCAGGcgggtgctg                                                                                                                                           >  1:468175/1‑118 (MQ=255)
                  aCCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCAGGcgggtgctg                                                                                                                                           <  2:468175/118‑1 (MQ=255)
                         tgGGTCGCGGGCATTCTCGCCCATCATACCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTg                                                                                                    <  1:424789/150‑1 (MQ=255)
                         ggggTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTg                                                                                                    <  1:534984/149‑1 (MQ=255)
                           ggTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGTACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGa                                                                                                  <  1:353764/150‑1 (MQ=255)
                                         tcGCCCATCGTGGCCATCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGaaa                                                                                    <  1:313624/150‑1 (MQ=255)
                                               aTCGTGGCCAGCTGGTTCCGGCCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGc                                                                              <  1:94797/150‑1 (MQ=255)
                                                tCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCg                                                                             >  1:225106/1‑150 (MQ=255)
                                                       cAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACACGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCaa                                                                      >  1:55865/1‑150 (MQ=255)
                                                              ttCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACg                                                               >  1:60208/1‑150 (MQ=255)
                                                                      aTCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAg                                                       >  1:270439/1‑150 (MQ=255)
                                                                          aCCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCt                                                   >  1:96548/1‑150 (MQ=255)
                                                                              tGCCGCCTTGAGCTTTGGACAGCCGATTCGGCGTCGCACCAGCACCAGCCTGGTACTCAAGTTTAATGCACACGTCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATgg                                               <  1:294221/150‑1 (MQ=255)
                                                                               gccgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATggg                                              <  2:225106/150‑1 (MQ=255)
                                                                                ccgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATgggg                                             <  2:60208/150‑1 (MQ=255)
                                                                                  gccTTGAGCTTTGCCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCGGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTg                                           <  1:456964/150‑1 (MQ=255)
                                                                                     ttGAGCTTTGGTCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGtt                                                                           >  1:471152/1‑115 (MQ=255)
                                                                                     ttGAGCTTTGGTCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGtt                                                                           <  2:471152/115‑1 (MQ=255)
                                                                                         gCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAg                                    >  1:91637/1‑150 (MQ=255)
                                                                                          cTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCGAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAgg                                   >  1:148761/1‑150 (MQ=255)
                                                                                          cTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAgg                                   >  2:396986/1‑150 (MQ=255)
                                                                                            ttGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCg                                 >  2:496978/1‑150 (MQ=255)
                                                                                                                   aCCAGCACCAGGCGGGTGCTGGTGCGCTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTACGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTg          >  1:439161/1‑150 (MQ=255)
                                                                                                                           ccGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTGCGCCAGGa  >  2:520879/1‑150 (MQ=255)
                                                                                                                            |                                                                                                                                                    
CAAGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTGCGCCAGGA  >  NC_002947/1693708‑1693980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑