| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,188,361 | G→T | 22.2% | L70I (CTA→ATA) | PP_5491 ← | SEC‑C motif domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,188,361 | 0 | G | T | 22.2% | 35.1 / 5.3 | 18 | L70I (CTA→ATA) | PP_5491 | SEC‑C motif domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base T (2/2); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
TGTAAAAGCGCTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAGCTTCTGGCGGATATGA > NC_002947/2188250‑2188498 | tGTAAAAGCGCTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTAtttt < 1:375988/150‑1 (MQ=255)tGTAAAAGCGCTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTAtttt < 1:95166/150‑1 (MQ=255) gcTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGaa < 1:144644/148‑1 (MQ=255) gcTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGaa > 2:144644/1‑148 (MQ=255) aCCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCg < 1:119850/134‑1 (MQ=255) aCCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCg > 2:119850/1‑134 (MQ=255) ccATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTAATAGTGGTAtgtgt > 1:484885/1‑150 (MQ=255) aTTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATa > 2:289440/1‑107 (MQ=255) aTTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATa < 1:289440/107‑1 (MQ=255) cAGTAGGAAGCTAAGCTGGATATCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCttt > 1:132750/1‑89 (MQ=255) cAGTAGGAAGCTAAGCTGGATATCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCttt < 2:132767/89‑1 (MQ=255) cAGTAGGAAGCTAAGCTGGATATCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCttt < 2:132750/89‑1 (MQ=255) cAGTAGGAAGCTAAGCTGGATATCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCttt > 1:132767/1‑89 (MQ=255) gTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAGCTTCTGGc > 2:452000/1‑150 (MQ=255) gctaagctGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAg > 1:60660/1‑135 (MQ=255) gctaagctGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAg < 2:60660/135‑1 (MQ=255) gctGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAGCTTCTGGCGGATATGa > 1:562551/1‑146 (MQ=255) gctGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAGCTTCTGGCGGATATGa < 2:562551/146‑1 (MQ=255) | TGTAAAAGCGCTTTAGCTGCCTAAGGCAATCCAGGGCGTCCTGTAGATTGCACCATGAGTCATGATATTTTTCGGCCTCTATGCATTTCCAGTAGGAAGCTAAGCTGGATAGCAACTCAAAGAAGCCGAAATAGATGAAGCAATTATTTTTCACGAAATCTTCGGCTGCACCAGCTTTGATATCGTTGATAGTGGTATGTGTGTTCTTGGCAAGCGTTAATGCATCGTCGTAGCTTCTGGCGGATATGA > NC_002947/2188250‑2188498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |