Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	794,134	C→T	A91A (GCC→GCT)	PP_0680 →	ATP‑dependent protease‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	794,134	0	C	T	100.0%	111.3 / NA	34	A91A (GCC→GCT)	PP_0680	ATP‑dependent protease‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

AACGTGCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCCGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGACGTTCCCGGCGGTGTTCGAACACC  >  NC_002947/793989‑794279                                                                                                                                                  |                                                                                                                                                  aaCGTGCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACt                                                                                                                                               >  1:696517/1‑150 (MQ=255)   cGTGCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTgg                                                                                                                                             <  2:677154/150‑1 (MQ=255)   cGTGCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTgg                                                                                                                                             <  2:215253/150‑1 (MQ=255)      gCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTc                                                                                                                                          <  2:133491/150‑1 (MQ=255)         gTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTAc                                                                                                                                       <  2:696517/150‑1 (MQ=255)         gTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTAc                                                                                                                                       >  1:812728/1‑150 (MQ=255)          ttGGGGCGCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGGCTGTGATGTGGGAGCCCGGCACCGGGCGCTTCTCGGTCGTCAAGCGCTAGCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACg                                                                                                                                      >  2:148188/1‑150 (MQ=255)                          tGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGt                                                                                                                                     <  2:300538/135‑1 (MQ=255)                          tGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGt                                                                                                                                     >  1:300538/1‑135 (MQ=255)                                                  ttACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTTGGTCTACGTCAACCACTTcg                                                                                                                          >  1:755680/1‑122 (MQ=255)                                                  ttACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTTGGTCTACGTCAACCACTTcg                                                                                                                          <  2:755680/122‑1 (MQ=255)                                                       aTGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGc                                                                                         >  2:474320/1‑150 (MQ=255)                                                        tgtgTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGcc                                                                                        >  2:849570/1‑150 (MQ=255)                                                            tATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTcc                                                                                    <  2:887351/150‑1 (MQ=255)                                                              tgtgATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCgg                                                                                  >  2:101604/1‑150 (MQ=255)                                                                                      gcgcTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGa                                                          >  1:95174/1‑150 (MQ=255)                                                                                        gcTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACa                                                        >  1:86654/1‑150 (MQ=255)                                                                                         cTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACAt                                                       <  2:760492/150‑1 (MQ=255)                                                                                            ctcGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCAATTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTtcatca                                                              >  1:352956/1‑140 (MQ=255)                                                                                            ctcGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCAATTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTtcatca                                                              <  2:352956/140‑1 (MQ=255)                                                                                               gttcgtCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCgg                                                 >  2:511634/1‑150 (MQ=255)                                                                                                      aaGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACAt                                                       <  1:412470/137‑1 (MQ=255)                                                                                                      aaGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACAt                                                       >  2:412470/1‑137 (MQ=255)                                                                                                      aaGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATc                                          <  2:812728/150‑1 (MQ=255)                                                                                                           tcaCCTCAATGCCGATGGGAAGCGCGAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACaa                                     <  2:790677/148‑1 (MQ=255)                                                                                                               cTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGAt                                               <  1:292589/136‑1 (MQ=255)                                                                                                               cTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGAt                                               >  2:292589/1‑136 (MQ=255)                                                                                                                 cAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACctgct                               <  2:95174/150‑1 (MQ=255)                                                                                                                      ccGAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGa                          <  1:849570/150‑1 (MQ=255)                                                                                                                        gAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACTTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGa                          >  2:236644/1‑148 (MQ=255)                                                                                                                        gAGGGCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACTTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGa                          <  1:236644/148‑1 (MQ=255)                                                                                                                            gCAAGCGCCAGCAGACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGACGTTcc                    >  2:333168/1‑150 (MQ=255)                                                                                                                                      gcagACCCCGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGACGTTCCCGGCGGTGtt          <  2:86654/150‑1 (MQ=255)                                                                                                                                              cGGCTGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGCATTGATCGACAACCTGCTGTCGACGTTCCCGGCGGTGTTCGAACAcc  <  1:474320/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AACGTGCTGTTGAGGCCCTGCAGTTTGGCGTGGCCATGCCGCGTCCTGGTTACAATGTGTATGTGATGGGCGAGCCCGGCACCGGGCGCTTCTCGTTCGTCAAGCGCTACCTCAAGGCCGAGGGCAAGCGCCAGCAGACCCCGGCCGACTGGGTCTACGTCAACCACTTCGACGACACCCGTGAACCACGTGCACTGGAGCTGCCGTCCGGAAGCGCCGCCGAGTTCATCAGCGACATGGGCGGATTGATCGACAACCTGCTGTCGACGTTCCCGGCGGTGTTCGAACACC  >  NC_002947/793989‑794279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑