Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,138,858	C→T	G265S (GGT→AGT)	arcB ←	ornithine carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,138,858	0	C	T	97.7%	136.1 / NA	45	G265S (GGT→AGT)	arcB	ornithine carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/23);  minor base G (1/0);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACGCTGGCGACTCGAACACGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACCGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACACCCTTGACCGCGGCTTTCGGGTCTTCG  >  NC_002947/1138715‑1139003                                                                                                                                                |                                                                                                                                                  gCACGCTGGCGACTCGAACACGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTCGGTTACAGGTgta                                                                                                                                             <  2:245820/150‑3 (MQ=255)     gCTGGCGACTCGAACACGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTtc                                                                                                                                         <  2:784749/150‑1 (MQ=255)           gACTCGAACACGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGt                                                                                                                                   >  1:882604/1‑150 (MQ=255)                  acacGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTgg                                                                                                                                                 <  2:926586/129‑1 (MQ=255)                      gtgaTCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTc                                                                                                                          >  1:977416/4‑148 (MQ=255)                      gTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTc                                                                                                                          <  2:977416/148‑1 (MQ=255)                      gTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCAc                                                                                                                        >  2:92574/1‑150 (MQ=255)                            ggTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATa                                                                                                                  >  1:236528/1‑150 (MQ=255)                              tCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAg                                                                                                                >  2:955850/1‑150 (MQ=255)                               cACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAgg                                                                                                               <  2:77668/150‑1 (MQ=255)                                  ttCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCtt                                                                                                            >  2:961386/1‑150 (MQ=255)                                   tCGATGCCGTTGGTCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTg                                                                                                           <  1:887403/150‑1 (MQ=255)                                     gATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAg                                                                                                         >  1:748124/1‑150 (MQ=255)                                         ccGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAgctgc                                                                                                     <  1:750203/150‑1 (MQ=255)                                         ccGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAgctgc                                                                                                     <  2:332608/150‑1 (MQ=255)                                              ggCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGAt                                                                                                <  2:882604/150‑1 (MQ=255)                                                ccAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATgc                                                                                              >  1:379321/1‑150 (MQ=255)                                                           tATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAg                                                                                   <  2:748124/150‑1 (MQ=255)                                                             ttGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGc                                                                                 <  1:92574/150‑1 (MQ=255)                                                                    gCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATc                                                                          <  2:236528/150‑1 (MQ=255)                                                                          tGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCg                                                                    >  2:219338/1‑150 (MQ=255)                                                                          tGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCg                                                                    <  1:219338/150‑1 (MQ=255)                                                                              tGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCa                                                                >  2:294056/1‑150 (MQ=255)                                                                                     cTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCt                                                                                                                      >  1:860624/1‑89 (MQ=255)                                                                                     cTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCt                                                                                                                      <  2:860624/89‑1 (MQ=255)                                                                                      ttGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATAcc                                                        >  1:916207/1‑150 (MQ=255)                                                                                                 gTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCTTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCTGCTCGCCCATCGATACCCACACGTCGGt                                             <  1:14299/150‑1 (MQ=255)                                                                                                     tgGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGc                                         >  2:752781/1‑150 (MQ=255)                                                                                                        aaTGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGc                                         >  2:779763/1‑147 (MQ=255)                                                                                                        aaTGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGc                                         <  1:779763/147‑1 (MQ=255)                                                                                                         aTGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGc                                                                                                              >  2:342414/1‑77 (MQ=255)                                                                                                         aTGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGc                                                                                                              <  1:342414/77‑1 (MQ=255)                                                                                                         aTGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACaa                                     <  1:294056/150‑1 (MQ=255)                                                                                                          tGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACaaa                                    >  1:827538/1‑150 (MQ=255)                                                                                                          tGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACaaa                                    >  2:601857/1‑150 (MQ=255)                                                                                                             cGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTc                                 <  2:827538/150‑1 (MQ=255)                                                                                                             cGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACACAAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTc                                 <  1:955850/150‑1 (MQ=255)                                                                                                             cGGCAGGCAATGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTc                                 <  1:534446/150‑1 (MQ=255)                                                                                                                  ggcagTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGATCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTAGacac                            >  1:750449/1‑150 (MQ=255)                                                                                                                      gTGCATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACACCCtt                        <  1:601857/150‑1 (MQ=255)                                                                                                                         cATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCtt                                                                               <  2:858988/92‑1 (MQ=255)                                                                                                                         cATGAACTTGGTCCGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCtt                                                                               >  1:858988/1‑92 (MQ=255)                                                                                                                                ttGGTCCGTGGGTTACGGGTGGAGTTCATCAATCCGGCATGTACCGGAGAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACAACCTTGAGCGCGGCt              >  2:162843/1‑150 (MQ=255)                                                                                                                                      cGTGGGTTACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACACCCTTGACCGCGGCTTTCggg        >  2:812030/1‑150 (MQ=255)                                                                                                                                            ttACTGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACACCCTTGACCGCGGCTTTCGGGTCTTCg  <  1:752781/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  GCACGCTGGCGACTCGAACACGTCATCGGTCACTTCGATGCCGTTGGCCAGGTCCGGGTATTGTTCGGCGATCTGCTTGCCTACCTTGGTTTCGGAGTTGTGGAATGCCGGCAGGCAGTGCATGAACTTGGTCCGTGGGTTACCGGTGGACTTCATCAGTTCGGCATTCACCTGATAAGGCTTGAGCTGCTTGATGCGCTCACCCCAGGCTTCGATCGGCTCGCCCATCGATACCCACACGTCGGTATGCACAAAGTCGACACCCTTGACCGCGGCTTTCGGGTCTTCG  >  NC_002947/1138715‑1139003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑