| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,852,053 | G→T | W366L (TGG→TTG) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,852,053 | 0 | G | T | 98.0% | 147.6 / ‑5.3 | 51 | W366L (TGG→TTG) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (26/24); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
TCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTG > NC_002947/1851909‑1852196 | tCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCTCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCt > 2:615940/1‑150 (MQ=255) cgacgaCTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTa < 1:750666/150‑1 (MQ=255) gacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACGGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAg < 1:28766/150‑1 (MQ=255) cgaCATCGCCAACCCCAAGGAGCACGGCTACCGCTCGTTGCACACCGCGTTAATCGGCCCCGAGGGCAAGGGGCTGGAGGAGCAGATCAGTGCCCACGGAATGCAAGAAGAGGCCGAACTTGGCGTATGCGCGCACTGGCGCTACAAggg > 2:265231/3‑150 (MQ=255) tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCAGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAAGTGCCGAACATAGCGAATGCGCCCCATTGCGCTACAAGGGc > 2:116588/1‑150 (MQ=255) tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGc > 2:693280/1‑150 (MQ=255) aTCGCCAACCCCAAGGAGAACGGCTACGGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCAcc > 1:578410/1‑150 (MQ=255) gCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGAc > 2:411239/1‑150 (MQ=255) ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGc > 2:649728/1‑150 (MQ=255) aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCa < 1:411239/150‑1 (MQ=255) ggAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAg > 1:941290/1‑150 (MQ=255) gaACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTc > 2:160413/1‑150 (MQ=255) cGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAGA‑AGATTTCCt < 1:160413/150‑1 (MQ=255) gTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTgg < 1:649728/150‑1 (MQ=255) aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaa‑a < 1:73306/138‑1 (MQ=255) aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaa‑a > 2:73306/1‑138 (MQ=255) aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGtt < 1:693280/150‑1 (MQ=255) aTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTg > 1:389220/1‑150 (MQ=255) ccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCACACGTTCGCTACAAGGGCACCGACGTCAAGACAACATCTAACTACCACTAAGAAA‑AGATTTCCTGGTTGCGTCAgg > 1:707612/1‑150 (MQ=255) gggCAAGGTGCTGGAGGCGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCt > 1:641776/1‑150 (MQ=255) gCAAGGTGCTGGATCTGCAGAGCCGTACCCACGGCATGCACGAAGAGGCCGAACTTTGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTgg < 2:389220/150‑1 (MQ=255) cAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGa < 1:318925/150‑1 (MQ=255) aaGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCAATTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAg > 2:359905/1‑150 (MQ=255) ggTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTg < 2:336143/150‑1 (MQ=255) tGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACg > 1:732152/1‑150 (MQ=255) gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt < 1:615940/150‑1 (MQ=255) gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt > 1:166490/1‑150 (MQ=255) gATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaa > 2:774100/1‑100 (MQ=255) gATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaa < 1:774100/100‑1 (MQ=255) aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc < 2:472107/150‑1 (MQ=255) ccACGGCATACACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg > 1:273791/1‑150 (MQ=255) cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGTACGAAGAGCTGGGCGACATCGGt < 1:670814/150‑1 (MQ=255) ggCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTg < 1:544936/101‑1 (MQ=255) ggCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTg > 2:544936/1‑101 (MQ=255) gAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAg > 1:663961/1‑150 (MQ=255) agagGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCa < 2:273791/150‑1 (MQ=255) gCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGGCGAAAGAGA‑TTCTTGTTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTg > 2:526937/1‑150 (MQ=255) cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTTGGCGACATCGGTGGCCTGGCCGAGCAGTTGCg > 2:176344/1‑150 (MQ=255) cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt > 2:377935/1‑130 (MQ=255) cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt < 1:377935/130‑1 (MQ=255) cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCg > 1:970217/1‑150 (MQ=255) cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCg > 2:234661/1‑150 (MQ=255) gAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg < 2:970217/150‑1 (MQ=255) aCTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGt < 2:732152/150‑1 (MQ=255) cTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc < 2:166490/150‑1 (MQ=255) aTGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGa < 1:639481/150‑1 (MQ=255) aTGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGa < 1:682344/150‑1 (MQ=255) gcgcCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGc < 2:641776/150‑1 (MQ=255) cgcCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGc < 1:972192/149‑1 (MQ=255) cgcCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCAATACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGc > 2:972192/1‑149 (MQ=255) cccACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTg > 2:164572/1‑150 (MQ=255) | TCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA‑AGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTG > NC_002947/1851909‑1852196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |