Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,656,397	A→C	L450R (CTG→CGG)	tktA ←	transketolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,656,397	0	A	C	100.0%	119.3 / NA	36	L450R (CTG→CGG)	tktA	transketolase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/16);  total (20/16)

TCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGT  >  NC_002947/5656253‑5656538                                                                                                                                                 |                                                                                                                                              tCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGcc                                                                                                                                          >  1:686591/1‑150 (MQ=255)   cACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGa                                                                                                                                        >  2:907083/1‑150 (MQ=255)   cACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGa                                                                                                                                        >  2:435114/1‑150 (MQ=255)     ccGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACa                                                                                                                                      >  1:775909/1‑150 (MQ=255)                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                   <  1:402160/150‑1 (MQ=255)                            tCCAGGTTCGGGGTGCTGCGCAGGCTGTTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGTGCACGGCGTTGCGGGCGTAt                                                                                                               >  2:228859/1‑150 (MQ=255)                                 gTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCAt                                                                                                          <  2:753324/150‑1 (MQ=255)                                            tgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGa                                                                                               <  1:618139/150‑1 (MQ=255)                                                    cTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTcgcg                                                                                       <  1:580379/150‑1 (MQ=255)                                                              gctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCa                                                                             >  2:823815/1‑150 (MQ=255)                                                                 cGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCa                                                                          <  2:686591/150‑1 (MQ=255)                                                                  gATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAg                                                                         <  2:281942/150‑1 (MQ=255)                                                                   aTCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAgg                                                                        >  2:576230/1‑150 (MQ=255)                                                                                 cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGc                                                          <  2:775909/150‑1 (MQ=255)                                                                                          ttCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGtt                                                 >  1:153066/1‑150 (MQ=255)                                                                                               ccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatga                                            <  1:823815/150‑1 (MQ=255)                                                                                               ccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatga                                            >  2:834316/1‑150 (MQ=255)                                                                                                 aGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAgg                                                                                            >  1:643341/1‑100 (MQ=255)                                                                                                 aGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAgg                                                                                            <  2:643341/100‑1 (MQ=255)                                                                                                 aGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatgatg                                          >  1:690799/1‑150 (MQ=255)                                                                                                     cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                      >  1:554937/1‑150 (MQ=255)                                                                                                          gAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCTCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATg                                 >  2:446387/1‑150 (MQ=255)                                                                                                                   gtgtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTg                                                                <  1:650103/110‑1 (MQ=255)                                                                                                                   gtgtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTg                                                                >  2:650103/1‑110 (MQ=255)                                                                                                                     gtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcgcg                      <  1:435114/150‑1 (MQ=255)                                                                                                                      tACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                              <  1:238475/77‑1 (MQ=255)                                                                                                                      tACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                              >  2:238475/1‑77 (MQ=255)                                                                                                                           gTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGcc                >  1:370401/1‑150 (MQ=255)                                                                                                                            tGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCg               >  2:231932/1‑150 (MQ=255)                                                                                                                                tCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCa                                                                          <  2:74111/87‑1 (MQ=255)                                                                                                                                tCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCa                                                                          >  1:74111/1‑87 (MQ=255)                                                                                                                                      gctgctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGaa          <  2:666169/145‑1 (MQ=255)                                                                                                                                      gctgctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGaa          >  1:666169/1‑145 (MQ=255)                                                                                                                                       ctgctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTa    <  1:907083/150‑1 (MQ=255)                                                                                                                                       cggctTCATCCGCGCCGACAGGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTa    <  2:370401/148‑1 (MQ=255)                                                                                                                                         gctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGt  >  2:109353/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                              TCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGT  >  NC_002947/5656253‑5656538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑