Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,741,273 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,741,2731.C80.0% 34.0 / 5.5 15intergenic (+464/+288)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (6/6);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741165‑4741409
                                                                                                             |                                                                                                                                           
cATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCCTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGctc                                                                                                  >  1:702371/1‑148 (MQ=255)
cATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGccc                                                                                                  >  1:294138/1‑150 (MQ=255)
       ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGacac                                                                                           <  1:739232/150‑1 (MQ=255)
           ttGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcc                                                                                       >  2:396403/1‑150 (MQ=255)
             gATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTCAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGtcctc                                                                                     <  2:484709/150‑1 (MQ=255)
                                                     ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             <  1:111399/150‑1 (MQ=255)
                                                        cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGACACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                          >  2:499215/1‑150 (MQ=255)
                                                                               ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                   <  1:396403/150‑1 (MQ=255)
                                                                                gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  >  2:510588/1‑150 (MQ=255)
                                                                                         tGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              <  1:406233/129‑1 (MQ=255)
                                                                                         tGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAA‑TCCAGACACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGcc         <  1:499215/150‑1 (MQ=255)
                                                                                         tGGTCACCCTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              >  2:406233/1‑129 (MQ=255)
                                                                                           gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             <  1:726159/112‑1 (MQ=255)
                                                                                           gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             >  2:726159/1‑112 (MQ=255)
                                                                                                cTTCGTGCTTGAGCAG‑CTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc  >  1:666756/1‑150 (MQ=255)
                                                                                                             |                                                                                                                                           
CATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741165‑4741409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑