Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,612	G→A	D384D (GAC→GAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,612	0	G	A	100.0%	69.7 / NA	23	D384D (GAC→GAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

ATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAGTT  >  NC_002947/1647471‑1647758                                                                                                                                              |                                                                                                                                                   aTCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATagag                                                                                                                                            >  2:299496/1‑150 (MQ=255)                             cGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgggcg                                                                                                                <  2:212040/150‑1 (MQ=255)                             cGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGCCCGCCTGGTCAACCAggccgccg                                                                                                                <  2:520861/150‑6 (MQ=255)                              ggATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGt                                                                                                               <  1:526470/150‑1 (MQ=255)                                                 gCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGtt                                                                                            >  2:445209/1‑150 (MQ=255)                                                                      cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                                       >  1:73835/1‑150 (MQ=255)                                                                        aGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATc                                                                                                                                                  >  1:39263/1‑73 (MQ=255)                                                                        aGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATc                                                                                                                                                  <  2:39263/73‑1 (MQ=255)                                                                          tGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGaaa                                                                   <  1:493508/150‑1 (MQ=255)                                                                            aaTGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCTAAAcc                                                                 >  2:514101/1‑150 (MQ=255)                                                                                     aTACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgtcgtc                                                        <  2:73835/150‑1 (MQ=255)                                                                                                            tgtCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTcc                                                       <  2:425656/128‑1 (MQ=255)                                                                                                            tgtCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTcc                                                       >  1:425656/1‑128 (MQ=255)                                                                                                                cTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgtcgt                                                         <  1:540466/122‑1 (MQ=255)                                                                                                                cTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgtcgt                                                         >  2:540466/1‑122 (MQ=255)                                                                                                                   cACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAgg                                                                         >  1:626855/1‑103 (MQ=255)                                                                                                                   cACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAgg                                                                         <  2:626855/103‑1 (MQ=255)                                                                                                                   cACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGaa                          <  1:445209/150‑1 (MQ=255)                                                                                                                         cAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCt                               >  1:690944/1‑139 (MQ=255)                                                                                                                         cAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCt                               <  2:690944/139‑1 (MQ=255)                                                                                                                               gCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctga              >  2:455904/1‑149 (MQ=255)                                                                                                                                           gTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCGCGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAGtt  >  1:471666/1‑150 (MQ=255)                                                                                                                                           gTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAGtt  >  1:246409/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                                   ATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATAGTT  >  NC_002947/1647471‑1647758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑