Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,242	G→T	E96* (GAG→TAG)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,242	0	G	T	100.0%	87.4 / NA	28	E96* (GAG→TAG)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/14);  total (14/14)

TAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTGG  >  NC_002947/1851096‑1851368                                                                                                                                                   |                                                                                                                               aagatAAAAAGGGCAACCCGTCCAAGCATTCCTGGGCGGATGCTACGTCCAGCTTTCAGTCAGGCCTGTAAATCGCGGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTAGCTGCGGTCATCTACCGCTCGGTGCGCTAgg                                                                                                                             <  2:94993/149‑1 (MQ=255)    agaAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCa                                                                                                                          >  2:198884/1‑150 (MQ=255)     gaAAAAGGGCAACGCGGCCAAGGATTCCTGGGCGGATGGTACGGACAGCTTCCAGGCAGGCCTGGAAGTCGCCGAAATTCTGGCTGACATCAAGCTCGACCAGGACTCCCTTGTGGCTGCGGTCATCTACCACTCGTTGCGCTAGGACaa                                                                                                                         >  2:50668/1‑150 (MQ=255)         aaGGGCAAACCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTc                                                                                                                     >  2:559191/1‑150 (MQ=255)                 cccGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGc                                                                                                             >  2:329974/1‑150 (MQ=255)                        aaGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAgg                                                                                                                             <  1:189422/127‑1 (MQ=255)                        aaGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAgg                                                                                                                             >  2:189422/1‑127 (MQ=255)                         aGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTca                                                                                                     <  1:640216/150‑1 (MQ=255)                                            tACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGTCTGACCTCAAGCTCGACCAGGACTCCCTGATGTCTGCGGTCATCTACCGATCGGTCCGTTAGGGCAAGGTCACCCTCGCCGAGTTCAGCCAGCGGTTTGCCCCggg                                                                                  >  2:333566/1‑149 (MQ=255)                                                 ccAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGgtgt                                                                             <  1:597337/150‑1 (MQ=255)                                                  cgatttCCAGGCCTGCCTGGAAATCGCCGAAATTCTGGCTGGCATGAAGCGCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTc                                                                            >  1:144289/5‑150 (MQ=255)                                                         caggcaggCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTc                                                                            >  1:386429/1‑143 (MQ=255)                                                         caggcaggCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTc                                                                            <  2:386429/143‑1 (MQ=255)                                                                gcCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACg                                                              <  1:559191/150‑1 (MQ=255)                                                                  cTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGgt                                                            <  1:329974/150‑1 (MQ=255)                                                                   tGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGgtg                                                           >  1:397171/1‑150 (MQ=255)                                                                                    cTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGgccgcc                                          <  1:32715/150‑1 (MQ=255)                                                                                           aCCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTg                                   <  1:198884/150‑1 (MQ=255)                                                                                             cTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGcc                                 >  1:706366/1‑150 (MQ=255)                                                                                             cTCAAGCTCGACCAGGACTCCCTGGTGCTTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGcc                                 >  1:511927/1‑150 (MQ=255)                                                                                                    tCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCa                          >  1:492853/1‑150 (MQ=255)                                                                                                     cGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAg                         <  1:33333/150‑1 (MQ=255)                                                                                                     cGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAg                         <  2:397171/150‑1 (MQ=255)                                                                                                            gACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtg                                                                                 >  2:452594/1‑87 (MQ=255)                                                                                                            gACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCggtg                                                                                 <  1:452594/87‑1 (MQ=255)                                                                                                              cTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGc                              <  2:475660/136‑1 (MQ=255)                                                                                                              cTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGc                              >  1:475660/1‑136 (MQ=255)                                                                                                              cTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCTAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACa                <  1:522599/150‑1 (MQ=255)                                                                                                                                                cgctAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTgg  >  2:61585/1‑130 (MQ=255)                                                                                                                                                   |                                                                                                                               TAGAGAAAAAGGGCAACCCGGCCAAGCATTCCTGGGCGGATGGTACGTCCAGCTTCCAGGCAGGCCTGGAAATCGCCGAAATTCTGGCTGACCTCAAGCTCGACCAGGACTCCCTGGTGGCTGCGGTCATCTACCGCTCGGTGCGCGAGGGCAAGGTCACCCTCGCCGAGGTCAGCCAGCGGTTTGGCCCGGTGGTGTCCAAGCTGATCGACGGTGTGCTGCGCATGGCCGCCATCAGTGCCAGCCTCAGCCCACGACAGTCGCTGGTGCTGG  >  NC_002947/1851096‑1851368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑