| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,188,015:1 | +A | 11.8% | intergenic (+76/‑134) | PP_3683 → / → PP_3684 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,188,015 | 1 | . | A | 11.8% | 112.1 / 12.2 | 34 | intergenic (+76/‑134) | PP_3683/PP_3684 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (16/14); new base A (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
TCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTG > NC_002947/4187870‑4188156 | tCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTa > 1:481153/1‑150 (MQ=255) tggttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCtt < 2:451975/150‑1 (MQ=255) ggttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑AGCAAGCGGCTTg < 1:670383/150‑1 (MQ=255) aCGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGc > 1:596436/1‑118 (MQ=255) aCGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGc < 2:596436/118‑1 (MQ=255) gATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCTTGGCCCGCCCCTGGCGGGGTTGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCg < 1:428804/150‑1 (MQ=255) gATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCt > 1:229385/1‑117 (MQ=255) gATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCt < 2:229385/117‑1 (MQ=255) gATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCg > 1:122040/1‑150 (MQ=255) cAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCaa > 2:394607/1‑150 (MQ=255) ggATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGc < 1:336843/150‑1 (MQ=255) tGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCTTg > 2:485282/1‑108 (MQ=255) tGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCTTg < 1:485282/108‑1 (MQ=255) ccTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCTAAACGGTGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATACTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGc < 1:609689/150‑1 (MQ=255) ccTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGc < 2:122040/150‑1 (MQ=255) aGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTc > 1:388496/1‑140 (MQ=255) aGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTc < 2:388496/140‑1 (MQ=255) ctcAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTCCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCa < 1:38397/150‑1 (MQ=255) tcGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGa > 1:277358/1‑150 (MQ=255) tcGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCGTGCCAAATCAAAAGCTCCTCTCTAGa > 1:120370/1‑150 (MQ=255) tCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTgc < 2:330545/103‑1 (MQ=255) tCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTgc > 1:330545/1‑103 (MQ=255) tCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGt > 1:134913/1‑150 (MQ=255) ccccTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTa < 2:205496/127‑1 (MQ=255) ccccTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTa > 1:205496/1‑127 (MQ=255) cccTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCg > 2:212589/1‑150 (MQ=255) ccTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGc < 1:394607/150‑1 (MQ=255) cGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGc > 2:445549/1‑150 (MQ=255) gCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGc > 1:530175/1‑150 (MQ=255) gCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGc > 1:393969/1‑150 (MQ=255) gTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCACCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCt < 2:278676/150‑1 (MQ=255) aaGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATACTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTg > 1:718318/1‑150 (MQ=255) aaGCCGCTT‑AGCAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAg > 1:176928/1‑140 (MQ=255) aaGCCGCTT‑AGCAAGCGGCTTGGCCCTAACGGGCTTTCATACTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAg < 2:176928/140‑1 (MQ=255) | TCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTG > NC_002947/4187870‑4188156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |