| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,541,821:1 | +G | 10.3% | coding (494/1503 nt) | nhaB → | Na(+)/H(+) antiporter NhaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,541,821 | 1 | . | G | 10.3% | 137.7 / 11.9 | 39 | coding (494/1503 nt) | nhaB | Na(+)/H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base . (21/14); new base G (2/2); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01 | |||||||||||
GTTCCTGTTCTCACGCATTTTGCTCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTG > NC_002947/4541672‑4541942 | gttcctgttcTCACGCATTTTGCTCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGGGCTGCGGTCGGCTTCTACGCGgt > 1:227432/1‑150 (MQ=255) aCGCATTTTGCTCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACcgcgt > 2:415545/1‑150 (MQ=255) aCGCATTTTGCTCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACcgcgt > 2:713147/1‑150 (MQ=255) tCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGGTA‑CACcgcgt < 1:10153/139‑1 (MQ=255) tCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGGTA‑CACcgcgt > 2:10153/1‑139 (MQ=255) tGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTcc > 2:732237/1‑140 (MQ=255) tGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTcc < 1:732237/140‑1 (MQ=255) cGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCcg > 2:577245/1‑150 (MQ=255) ttCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTATACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCcgcg > 1:61751/1‑150 (MQ=255) tCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCcgcgc < 1:415545/150‑1 (MQ=255) cAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGa < 1:577245/150‑1 (MQ=255) aGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAgg > 2:4040/1‑150 (MQ=255) ttCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCggg < 1:641403/128‑1 (MQ=255) ttCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCggg > 2:641403/1‑128 (MQ=255) ctgTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGc > 1:309665/1‑150 (MQ=255) ttGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCa < 1:634646/150‑1 (MQ=255) gCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCAc < 1:4040/150‑1 (MQ=255) gCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCAc > 1:195591/1‑150 (MQ=255) tCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCgg > 2:59553/1‑150 (MQ=255) cgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGTCGTCTACGCGGT‑GTACCACCGCGTCGCGTCCCGGGCCAATCCGCGCGAGGACAGCGCACTGAAGAGCGACCAGCAAGTCGCTCACTTGCCCCGGGAAg > 2:156270/1‑150 (MQ=255) tcGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCg > 1:690157/1‑150 (MQ=255) gATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGa > 1:444281/1‑113 (MQ=255) gATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGa < 2:444281/113‑1 (MQ=255) ccTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGtt > 1:447706/1‑150 (MQ=255) tGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTcc > 1:372829/1‑150 (MQ=255) atcGTCACGGCGGTGATCAACAGCGCTGCGGGCGGCTTCTACGCGGC‑GTACCACCGCGTCGCGTCCGGAGCCAATCCGCGCGAGGACAGCCCACCGGAGCGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCcga < 2:126912/148‑2 (MQ=255) ccGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCcgcg < 1:713147/150‑1 (MQ=255) gTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGcc > 1:517986/1‑150 (MQ=255) cggcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTcc < 2:517986/150‑1 (MQ=255) ggcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCGGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCt > 2:128207/1‑150 (MQ=255) gcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAg > 2:90840/1‑75 (MQ=255) gcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAg < 1:90840/75‑1 (MQ=255) gcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTg > 2:10585/1‑150 (MQ=255) cggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGc < 2:317849/150‑1 (MQ=255) cggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGc < 1:128207/150‑1 (MQ=255) atcaGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctg > 1:558636/1‑150 (MQ=255) ttCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCg < 2:426531/79‑1 (MQ=255) ttCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCg > 1:426531/1‑79 (MQ=255) gcgaCGCGGTGGTA‑CACCGCGTAGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAc < 2:636522/101‑1 (MQ=255) gcgaCGCGGTGGTA‑CACCGCGTAGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAc > 1:636522/4‑104 (MQ=255) | GTTCCTGTTCTCACGCATTTTGCTCGGGGTGCGTTCCAAGGCCATTCTGGCGCTGCTGTTTTGCGTACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGT‑GTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTG > NC_002947/4541672‑4541942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |