Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,468,121:1	+A	9.5%	coding (819/1047 nt)	mltB ←	membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,468,121	1	.	A	9.5%	155.4 / 11.8	42	coding (819/1047 nt)	mltB	membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base . (17/21);  new base A (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

GAGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATG  >  NC_002947/5467973‑5468270                                                                                                                                                       |                                                                                                                                                      gAGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑t                                                                                                                                                      >  2:279102/1‑150 (MQ=255)  aGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑Tc                                                                                                                                                     >  1:506624/1‑150 (MQ=255)       tCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGc                                                                                                                                                <  2:377492/150‑1 (MQ=255)         gcgTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCg                                                                                                                                              <  2:506624/150‑1 (MQ=255)            tAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtg                                                                                                                                           <  2:470808/150‑1 (MQ=255)                  ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAgg                                                                                                                                     >  1:139572/1‑150 (MQ=255)                     ttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCt                                                                                                                                  <  2:329940/150‑1 (MQ=255)                               tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgc                                                                                                                        >  2:268479/1‑150 (MQ=255)                                  aGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCt                                                                                                                     >  2:591692/1‑150 (MQ=255)                                    tACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCgg                                                                                                                                             <  1:231527/124‑1 (MQ=255)                                    tACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCgg                                                                                                                                             >  2:231527/1‑124 (MQ=255)                                      cTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCAcc                                                                                                                 >  2:280173/1‑150 (MQ=255)                                          gggCCGCCCACGCCGTCGACACGGAAGGCGGTAACCGGCAGATCATCGCGCAGAGCACCA‑GCGCGGAGGCCGCGAAGCCCACGCACCTCCCCCACCGTTTTCACCGGG‑TCGATGCCGGTGTTCAGGCCTTCGTCGGCGCGCTCACCTTcc                                                                                                             <  1:733641/150‑1 (MQ=255)                                            gCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCAc                                                                                                           <  1:279102/150‑1 (MQ=255)                                             ccGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCAcc                                                                                                          <  1:399581/150‑1 (MQ=255)                                               gCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCa                                                                                                        >  2:722300/1‑150 (MQ=255)                                               gCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCa                                                                                                        >  2:78468/1‑150 (MQ=255)                                                          cGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGa                                                                                             <  1:268479/150‑1 (MQ=255)                                                             gaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACca                                                                                          <  1:280173/150‑1 (MQ=255)                                                                ggAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac                                                                                       >  1:642911/1‑150 (MQ=255)                                                                 gAAGGCGGTAACCGGCAGATCATCTCGCAGCAAATCATGAACCGA‑CCAGCCAAGCGTTCGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACAcc                                                                                      <  1:625372/150‑1 (MQ=255)                                                                       ggTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                       <  2:398936/95‑5 (MQ=255)                                                                       ggTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                       >  1:398936/1‑91 (MQ=255)                                                                           aCCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTAGTGCTGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGcc                                                                            <  1:422573/150‑1 (MQ=255)                                                                           aCCGGCAGATCATCGCGCAGCGAATCATGAACAGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGcc                                                                            >  2:86069/1‑150 (MQ=255)                                                                                                     aTGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTa                                                     >  1:442424/1‑147 (MQ=255)                                                                                                     aTGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTa                                                     <  2:442424/147‑1 (MQ=255)                                                                                                        aaCCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGc                                               >  2:197906/1‑150 (MQ=255)                                                                                                         aCCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGcc                                              >  2:236333/1‑150 (MQ=255)                                                                                                                cAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATg                                                                                                                                                 >  2:2774/1‑45 (MQ=255)                                                                                                                cAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATg                                                                                                                                                 <  1:2774/45‑1 (MQ=255)                                                                                                                     aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTc                                                                                   >  1:380836/1‑102 (MQ=255)                                                                                                                     aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTc                                                                                   <  2:380836/102‑1 (MQ=255)                                                                                                                     aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt                                   <  2:642911/150‑1 (MQ=255)                                                                                                                                aCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCTCCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAg                        <  1:384520/150‑1 (MQ=255)                                                                                                                                aCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAg                        <  1:86069/150‑1 (MQ=255)                                                                                                                                  tCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATgg                                 >  2:239927/1‑139 (MQ=255)                                                                                                                                  tCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATgg                                 <  1:239927/139‑1 (MQ=255)                                                                                                                                     ccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttg                   >  1:592300/1‑150 (MQ=255)                                                                                                                                        aCCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTc                <  2:139572/150‑1 (MQ=255)                                                                                                                                            ttttCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGa            <  2:424754/150‑1 (MQ=255)                                                                                                                                              accACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTc                                                                                                              >  2:525414/3‑50 (MQ=21)                                                                                                                                              accACCGGCATCGA‑GCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTc                                                                                                              <  1:525414/48‑1 (MQ=21)                                                                                                                                                      c‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATg  <  1:78468/150‑1 (MQ=255)                                                                                                                                                      c‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATg  <  1:197906/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      GAGTGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGA‑CCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATG  >  NC_002947/5467973‑5468270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑