| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,479,987:1 | +G | 15.7% | coding (463/993 nt) | prmA → | ribosomal protein L11 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,479,987 | 1 | . | G | 15.7% | 159.1 / 30.3 | 51 | coding (463/993 nt) | prmA | ribosomal protein L11 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (23/20); new base G (4/4); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
AACAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACTT > NC_002947/5479838‑5480129 | aaCAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCATTACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCt < 1:279332/150‑1 (MQ=255)aaCAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCATCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCt > 1:435675/1‑150 (MQ=255) gtTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGa < 2:543655/150‑1 (MQ=255) gtTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGa > 1:181632/1‑150 (MQ=255) gCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGcc > 1:175066/1‑150 (MQ=255) gcgcCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGaaaa > 2:459845/1‑150 (MQ=255) cgcCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAg < 1:567018/150‑1 (MQ=255) cGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGaa > 1:171582/1‑150 (MQ=255) gcgcCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGcc < 2:503769/128‑1 (MQ=255) gcgcCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGcc > 1:503769/1‑128 (MQ=255) aGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCtgctgc > 1:722183/1‑150 (MQ=255) aGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTg < 2:315108/150‑1 (MQ=255) tGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCgg > 2:557333/1‑135 (MQ=255) tGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCgg < 1:557333/135‑1 (MQ=255) ggACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCAc > 1:277490/1‑150 (MQ=255) gACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCCGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGAGGGCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACt > 1:609464/1‑150 (MQ=255) ccAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCa < 2:175066/150‑1 (MQ=255) cAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGaaaa > 2:667258/1‑95 (MQ=255) cAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGaaaa < 1:667258/95‑1 (MQ=255) cAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAc < 2:171582/150‑1 (MQ=255) cAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAc > 1:445651/1‑150 (MQ=255) gACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCAcccc > 2:89278/1‑150 (MQ=255) gACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAAGACGCCGTGAACCTGCTGCTc < 1:154980/114‑1 (MQ=255) gACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAAGACGCCGTGAACCTGCTGCTc > 2:154980/1‑114 (MQ=255) tGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCccacc > 1:518315/1‑150 (MQ=255) gAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACg > 2:340037/1‑150 (MQ=255) aGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt > 1:182804/1‑69 (MQ=38) aGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt < 2:182804/69‑1 (MQ=38) aGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt > 1:182688/1‑69 (MQ=38) aGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTG‑CCAGCTGGGCACGAt < 2:182688/69‑1 (MQ=38) cAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATg > 2:667945/1‑150 (MQ=255) aaCTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATgg < 1:667945/150‑1 (MQ=255) aaCTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATgg > 2:618723/1‑150 (MQ=255) aaCTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATgg < 2:181632/150‑1 (MQ=255) ttCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTgg < 1:12689/107‑1 (MQ=255) ttCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTgg > 2:12689/1‑107 (MQ=255) ttCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTc > 1:726551/1‑150 (MQ=255) ccAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGa < 2:726551/150‑1 (MQ=255) aGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACg < 2:277490/150‑1 (MQ=255) gccgccgccTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAg < 1:89278/150‑1 (MQ=255) ggggcaTCGTG‑CCAGCTGGGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCt < 1:347375/101‑1 (MQ=255) ggggcaTCGTG‑CCAGCTGGGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCt > 2:347375/6‑106 (MQ=255) gtgGATCGTGCACAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCGGGt > 1:667142/1‑150 (MQ=255) tgGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGTCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTg < 1:459845/150‑1 (MQ=255) tgGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTg > 1:315937/1‑150 (MQ=255) aTCGTG‑CCAGCTGGGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCtgt < 2:109355/103‑1 (MQ=255) aTCGTG‑CCAGCTGGGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCtgt > 1:109355/1‑103 (MQ=255) cGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGa < 1:5199/150‑1 (MQ=255) gCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACtt < 2:722183/150‑1 (MQ=255) ccAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCagcagc < 2:101897/123‑1 (MQ=255) ccAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCagcagc > 1:101897/1‑123 (MQ=255) cT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCcagca < 2:113125/117‑1 (MQ=255) cT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCcagca > 1:113125/1‑117 (MQ=255) | AACAGGTGTTCGCCCACCTGCGCCTGCTGACCGGCGCCGAGCTGCCCGAGCACCAGGCCGAGGTGATCGAGGACCAGGACTGGGAACGCAGCTGGATGGACAACTTCCAGCCGATGCGCTTCGGCCGCCGCCTGTGGATCGTGCCCAGCT‑GGCACGATGCCCCGGAAAAGGACGCCGTGAACCTGCTGCTCGACCCGGGCCTGGCCTTTGGCACCGGCACTCACCCCACCACGGCCCTGTGCCTGGAATGGCTCGACGGCCAGCAGCTTGAAGGCACCCAGGTGCTGGACTT > NC_002947/5479838‑5480129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |