| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,925,635:1 | +C | 9.5% | coding (566/1083 nt) | gcvT‑II ← | aminomethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,925,635 | 1 | . | C | 9.5% | 152.9 / 11.7 | 42 | coding (566/1083 nt) | gcvT‑II | aminomethyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (20/18); new base C (2/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
CTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGA > NC_002947/5925490‑5925781 | cTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGTGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑tata > 2:182494/1‑150 (MQ=255) aGGTTCATGCCGGCTTCCAGCCGCAGGGTACCCCGGGCGCCCAGGCCGCTTGGCGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGACGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCgg < 1:714836/150‑1 (MQ=255) ggTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg < 2:251488/150‑1 (MQ=255) gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGGCTATA‑CCGGTGAgga > 1:65938/1‑148 (MQ=255) gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGGCTATA‑CCGGTGAgga < 2:65938/149‑2 (MQ=255) ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAc > 2:492723/1‑150 (MQ=255) cGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAcc > 1:114957/1‑150 (MQ=255) cGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAcc > 2:379210/1‑150 (MQ=255) tCCAGGGGCAGGGTATCGCGGCCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGGCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg < 2:287518/150‑1 (MQ=255) gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTc < 1:245818/150‑1 (MQ=255) aGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCa > 1:431123/1‑150 (MQ=255) gcgcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAAc < 2:279983/150‑1 (MQ=255) ccAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGgc > 2:35892/1‑150 (MQ=255) aGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGgcgc > 2:607798/1‑150 (MQ=255) gCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGGCTATA‑CCGGTgaggac < 1:525593/105‑6 (MQ=255) gCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGGCTATA‑CCGGTgaggac > 2:525593/1‑100 (MQ=255) ttGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGCCGACGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgc > 1:7039/1‑150 (MQ=255) ttGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCa > 2:415509/1‑112 (MQ=255) ttGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCa < 1:415509/112‑1 (MQ=255) ttGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgc < 1:379210/150‑1 (MQ=255) ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt < 2:141216/150‑1 (MQ=255) cGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGa < 2:505414/150‑1 (MQ=255) tACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTg > 2:66089/1‑150 (MQ=255) ccAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCgg < 2:114957/150‑1 (MQ=255) aagaagGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgc > 2:742686/1‑118 (MQ=255) aagaagGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgc < 1:742686/118‑1 (MQ=255) aagaagGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAgg < 2:173661/150‑1 (MQ=255) agaagGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGc < 1:311929/66‑1 (MQ=255) agaagGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGc > 2:311929/1‑66 (MQ=255) ccACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGCGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCa > 1:423403/1‑150 (MQ=255) aCCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAcc > 2:92092/1‑150 (MQ=255) aCCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAcc > 1:148264/1‑150 (MQ=255) tCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCgg < 1:66089/150‑1 (MQ=255) tCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCgg < 1:607798/150‑1 (MQ=255) gatgatCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGccc > 1:320743/1‑150 (MQ=255) cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGa > 1:707705/1‑107 (MQ=255) cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGa < 2:707705/107‑1 (MQ=255) cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGc > 2:577606/1‑150 (MQ=255) aGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCa < 2:745847/150‑1 (MQ=255) gCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAg < 1:500052/150‑1 (MQ=255) cGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGa > 2:485286/1‑150 (MQ=255) aCCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAg > 2:319234/1‑150 (MQ=255) tcGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGa > 2:639993/2‑150 (MQ=255) | CTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCCAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGA > NC_002947/5925490‑5925781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |